55 88 Medium Kit Without Serum and Without Growth Factors
Medium Kit Without Serum and Without Growth Factors
Kit consists of 500mL 4Z3-500-S Medium and 10mL Attachment Factor™. This is not a complete medium. For complete medium, add 10 - 20% Serum (Defined FBS or equivalent) and animal derived growth factor, CultureBoost™ (4CB-500) or human recombinant growth factor, CultureBoost-R™ (4CB-500-R). 4Z3-500-S is Certified and intended for experimental application. Cell Systems media and reagents are sterile, made with WFI and all components are cGMP and ISO Compliant.
Available in one 500 mL container.
Certified for use with more than 100 human and animal cell cultures and cell lines including these Cell Systems Certified cells:
Bone Marrow Cells (CFU-F and Erythroid Burst Cultures)
Connective Tissue Cells
Dermal Fibroblast Cells
Endothelial Cells (Aortic, Arterial, Coronary Artery, Vascular and Venous)
Fetal Lung Cells
Embryonic and Adult Stem Cells
Glomerular Mesangial Cells
Immortalized / Tumor-derived Mesenchymal Cells
Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal)
Smooth Muscle Cells
Tumor Neovascular Cells
Bovine and Porcine large vessel and microvessel Endothelial Cells
Characteristics of Medium formulated without Serum
Cell Systems Medium contains no added hormones.
Medium contains no antibiotics; however, Bac-Off®, (Cat #4Z0-643), is the recommended antibiotic for all Cell Systems medium. Bac-Off® is at or above the [2ng/mL] MIC of aerobic gram-positive and gram-negative microorganisms. (See Bac-Off® certificate.)
Medium contains no phenol red. Phenol red in cell culture medium binds to receptors on cell surfaces and causes the cell to respond as though pharmacologic levels of estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) are present in the medium. We exclude this unnecessary additive in order to optimize experimental conditions for researchers.
Cell Systems Medium is shipped at ambient temperature. If the entire unit will not be used within 30 days, activate the medium with the enclosed supplement then aliquot and freeze in smaller units which will be used within 30 days.
Upon receipt, if the full 500mL medium unit will be used within 30 days, immediately refrigerate the medium and supplements. Once the unit is activated, or any component of the medium kit is opened, the shelf life is 30 days at refrigerated (4 - 8°C) temperatures.
- "Insulin and beta-adrenergic Receptors Inhibit Retinal Endothelial Cell Apoptosis Through Independent Pathways" Panjala and Steinle Neurochemical Research, 2010.
- "Sympathetic nuerotransmission modulates expression of inflammatory markers in the rat retina" Steinle Experimental Eye Research, 2007.