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                Load image into Gallery viewer, Template 2 Page of Primary Human Retinal Microvascular Endothelial Cells (ACBRI 181)
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Template 2 Page of Primary Human Retinal Microvascular Endothelial Cells (ACBRI 181)

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  • -$-735.00
  • Regular price $735.00
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    Cell Systems ACBRI 181 Primary Human Microvascular Endothelial Cells

    Primary Human Retinal Microvascular Endothelial Cells (ACBRI 181) are initiated by elutriation of dispase dissociated normal human retinal tissue.

    Cell Initiation 

    These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm² flask. Each vial contains more than 1 x 10cells.

    In addition to cryopreserved vial, these cells are also available in 25cm² and 75cm² proliferating cell culture flasks (US domestic market only).

    Click For Current Applications and Assays Using Cell Systems Primary Human Retinal Microvascular Endothelial Cells (ACBRI 181)

    Companion Products 

    Each vial of cells is shipped to customer with Bac-Off® (antibiotic), and CultureBoost™ (animal derived growth factors) or CultureBoost-R (human recombinant growth factors)

    These cells are qualified for use with:

    New Column

    Characterization in Peer-Reviewed Literature

    Cell Systems Primary Human Retinal Microvascular Endothelial Cells (HREC) express hallmark endothelial cell markers such as PECAM1/CD31 (A), von Willebrand Factor (B), and VE-Cadherin/CDH5 (C); and treatment with TNF-alpha induces expression of endothelial-cell specific protein E-selectin (D) as measured by mRNA abundance. In contrast, the widely-used immortalized cell line RF/6A does not share hallmark endothelial cell characteristics. Modified with permission from Figures 1-3, Makin et al. IOVS (2018) 59:5795-5802.

    Citations:

     

    • Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.

    Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.

    Please read our Terms and Conditions

    Cell Systems human primary cells were isolated by elutriation of dispase dissociated normal human retinal tissue without the use of positive selection of antibodies.  Learn More

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    Cell Systems human primary cells were isolated by elutriation of dispase dissociated normal human retinal tissue without the use of positive selection of antibodies. Antibody-free primary cells can offer a more biologically relevant cell culture tool for researchers.

     

    Enhance insights with increased biological relevance

    These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are crypopreserved in Cell Systems Cell Freezing Medium (4Z0-705).

     

    • Available at Passage 3 (<12 cumulative population doublings)
    • Each vial contains approximately 1 x 106 cells
    • Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
    • Available in reserved lots to enhance consistency in your research program

     

    Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.

     

    CIATATIONS

     

    TESTS


    Standard Tests


    TESTS RESULTS

    HIV Serologic Test (donor level HIV AB EIA)

    Negative

    HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab)

    Negative

    Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level)

    Negative

    Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)

    Negative


    Miscellaneous Tests


    TESTS RESULTS

    Cytoplasmic VWF / Factor VIII

    > 95% positive by immunofluorescence

    Cytoplasmic uptake of Di-I-Ac-LDL

    > 95% positive by immunofluorescence

    Cytoplasmic CD31

    > 95% positive by immunofluorescence

    GFAP (Glial Fibrillary Acidic Protein)

    < 1% by immunofluorescence

    Glutamine Synthetase

    < 1% by immunofluorescence

    NG2 (Neural/Glial Antigen 2)

    < 1% by immunofluorescence

    PDGFR-beta (Platelet Derived Growth Factor Receptor beta)

    < 1% by immunofluorescence

    PROTOCOLS

    Optimize your research with human primary cells.

    Avoid the limitations from cell lines or animal models with more timely insights into human cell biology.

    COMPANION PRODUCTS:

    The System™ (CSS-A101) Complete Cell Culture Kit

    Passage Reagent Group™ (4Z0-800)