Attachment Factor™ promotes cell attachment to tissue culture surfaces

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Primary Human Glomerular Mesangial Cells (ACBRI 127)

Gain Consistency with our Single Lot Media and Reagents


Attachment Factor™ (4Z0-210) is an extracellular matrix (ECM) product that promotes cell attachment to tissue culture surfaces and encourages correct polarity and cytoskeletal organization. Use of Attachment Factor™ is critically important when cultures are to be initiated, grown, passaged, or used within the Cell Systems Media family.

The biologically active elements of Attachment Factor™ are derived by a chromatographic process from heat-inactivated charcoal-stripped fetal bovine serum, and delivered at 1X in a PBS-based HEPES-buffered gelatin vehicle. Cell Systems exclusively uses and strongly recommends the use of serum products 100% traceable to cattle processed in the US at USDA inspected facilities.



Use of Attachment Factor™ improves plating efficiency, shortens "lag time" after passage, enhances growth rate, and promotes the organization of polarity and cytoskeletal architecture, and can dramatically increase cell yield during cell culture expansion. Use with all cultures except Melanocyte and Keratinocyte cells.

Without Attachment Factor

Primary Human Brain Microvascular Endothelial Cell (ACBRI 376)

With Attachment Factor

Primary Human Brain Microvascular Endothelial Cell (ACBRI 376)

A Selection of Citations for 4Z0-201 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems Attachment Factor™.



Storage and Handling

Store Attachment Factor™ at 2-8°C. Once product is opened, shelf life is 30 days at refrigerated temperatures (2-8°C). Warm to 37°C in water bath before each use. Refrigerate immediately after use.

For use of Attachment Factor™ in passaging and plating of cells, please see our FAQ section. 

Cell Systems media and reagents are made with WFI, all components are cGMP and ISO Compliant, and are classified "Sterile".


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Using Cell Systems optimized media and reagent kits can remove variables from your research program while enhancing consistency.