3D MODELING
Cell Systems can provide your research institute with an unmatched level of consistency that is required for optimized 3D culture experiments.
Try Cell Systems primary cells in research that utilizes:
CRISPR RESEARCH
Immortalized cells may hinder relevant insights in your CRISPR research --providing inauthentic results not aligned with the natural state of cells.
Try using Cell Systems primary cells in your CRISPR research for:
Preliminary
Data
Save time by eliminating less relevant research
Model
Organism
Primary cells provide real human insights.
Therapeutic
Breakthrough
Increase speed to discovery
Selection of assays in which our primary cells have been utilized:
3D Culture
Adhesion
Apoptosis
Calcium Release
Caspase Activity
Cell Cycle
Cellular Metabolism
Co-Culture
Comet Assay
Flow Cytometry
Gelatin zymography
Glycosylation Profiling
Immunocytochemistry
Immunoprecipitation
Invasion/Migration
Mitochondrial Membrane Potential
Monolayer Permeability
Next-Gen Sequencing
Neutrophil Adhesion
Parasite Binding
Proliferation and Viability
Reactive Oxygen
Species RNAi RT-PCR
Sequencing RT-PCR
Subcellular Fractionation
Scanning EM
Transient Transfection
Tube Formation
Viral Transduction
Western Blot
Cell Systems primary cells are:
3D cell culture is an exciting new paradigm in basic and clinical biomedical research. Culturing cells in three-dimensional space – on a scaffold, in a matrix, or within a bioreactor – offers certain advantages over the conventional monolayer model. Most importantly, the 3D environment better recapitulates the cell’s natural context in the human body. Researchers are finding that 3D cell culture and co-culture (combinations of defined cell types in close proximity) allow for better prediction of drug activity and toxicity, and stronger ex vivo models of biological processes and cellular mechanisms (Ref 1-4).
In contrast to immortalized cell lines, Cell Systems Primary Human Cells are isolated directly from human tissue, without genetic modification or manipulation. Most of our cell isolates are purified through a unique process which yields pure populations of cells unadulterated by antibody labels. Our deep inventory of high-purity primary cells translates to more consistent, higher quality, and more meaningful results from your work.
Brown JA, Pensabene V, Markov DA, Allwardt V, Neely MD, Shi M, Britt CM, Hoilett OS, Yang Q, Brewer BM, Samson PC, McCawley LJ, May JM, Webb DJ, Li D, Bowman AB, Reiserer RS, Wikswo JP. Biomicrofluidics. 2015 Sep; 9(5): 054124. doi: 10.1063/1.4934713
Figure 2. Live/dead stain of cell culture within the NeuroVascular Unit (NVU). (a) Human brain-derived microvascular endothelial cells (Cell Systems ACBRI 376) in the perfusion channel of the NVU. Live = green; dead = red (DIV 14; DIV = days in vivo). (b) Endothelial cells coating the vascular chamber (DIV 14). (c) Pericytes and astrocytes on the membrane in the brain chamber. (d) Neurons in the brain chamber suspended in collagen gel (DIV 14). (e) Endothelial cells in the perfusion channels of the NVU (DIV 21). (f) Live/dead and (g) live-only staining of endothelial cells coating the vascular chamber side of the membrane (DIV 21). Over 80% viability is maintained after 3 weeks in culture (DIV 21, live only). (h) Pericytes and astrocytes on the brain side of the same membrane region as in (g) and (h), with the collagen shrinkage shown. Modified from original publication with permission of authors; distributed under Creative Commons license.
Herland A, van der Meer AD, FitzGerald EA, Park T-E, Sleeboom JJF, Ingber DE. PLoS ONE. 2016; 11(3): e0150360. doi: 10.1371/journal.pone.0150360
Excerpt from Figure 1. Low magnification micrograph of an entire microfluidic device containing a lumen filled with blue fluid (bar, 3 mm).
Excerpt from Figure 2. Fluorescence confocal micrographs of the engineered brain microvessel viewed from the top. The fluorescence micrographs show the cell distributions in 3D BBB chips containing brain microvascular endothelium (Cell Systems ACBRI 376) with prior plating of brain pericytes (Cell Systems ACBRI 499) on the surface of the gel in the central lumen (D) or endothelium with brain astrocytes embedded in the surrounding gel (G) (bars, 200 μm). Green indicates F-actin staining, blue represents Hoechst-stained nuclei, and magenta corresponds to VE-Cadherin staining, except for G where morphology and intensity masks were used to discriminate astrocytes (green) from endothelial cells (magenta). Modified from original publication with permission of authors; distributed under Creative Commons license.
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