Attachment Factor™ - Cell Systems

Attachment Factor™ promotes cell attachment to tissue culture surfaces

Attachment Factor™ (4Z0-210) is an extracellular matrix (ECM) product that promotes cell attachment to tissue culture surfaces and encourages correct polarity and cytoskeletal organization. Use of Attachment Factor™ is critically important when cultures are to be initiated, grown, passaged, or used within the Cell Systems Media family. Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.

The biologically active elements of Attachment Factor™ are derived by a chromatographic process from heat-inactivated charcoal-stripped fetal bovine serum, and delivered at 1X in a PBS-based HEPES-buffered gelatin vehicle. Cell Systems exclusively uses and strongly recommends the use of serum products 100% traceable to cattle processed in the US at USDA inspected facilities.

Purpose

Use of Attachment Factor™ improves plating efficiency, shortens "lag time" after passage, enhances growth rate, and promotes the organization of polarity and cytoskeletal architecture, and can dramatically increase cell yield during cell culture expansion. Use with all cultures except Melanocyte and Keratinocyte cells.

Instructions for Use:

See the Attachment Factor Protocol.

Handling and Storage

Store Attachment Factor™ at <-20°C. Once product is opened, shelf life is 30 days at refrigerated temperatures (2-8°C). Warm to 37°C in water bath before each use. Refrigerate immediately after use.

For use of Attachment Factor™ in passaging and plating of cells, please see our FAQ section. 

Companion Products

Citations

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• "Immune complexes suppressed autophagy in glomerular endothelial cells"  Wang & Law, Cellular Immunology, 2018

• "Effect of endoplasmic reticulum stress mediated by hyperglycemia on barrier function in retinal cells"  Saif, (Dissertation) 2016

• "Interaction between endothelial protein C receptor and intercellular adhesion molecule 1 to mediate binding of Plasmodium falciparum-infected erythrocytes to endothelial cells" Avril et al. mBio, 2016

• "Different regulation of p53 expression by cadmium exposure in kidney, liver, intestine, vasculature, and brain astrocytes"  Lee et al. Toxicology Research, 2016

• "Distinct contributions of astrocytes and pericytes to neuroinflammation identified in a 3D human blood-brain barrier on a chip"  Herland et al, PLoS One, 2016

• "Optimization of an in vitro human blood–brain barrier model: Application to blood monocyte transmigration assays"  Paradis et al. MethodsX, 2015

• "A2A adenosine receptor regulates the human blood brain barrier permeability"  Kim and Bynoe, Molecular Neurobiology, 2014

• "Differential expression and role of hyperglycemia induced oxidative stress in epigenetic regulation of β1, β2 and β3-adrenergic receptors in retinal endothelial cells"  Safi et al. BMC Medical Genomics, 2014

• "Cycloviolacin O2 (CyO2) suppresses productive infection and augments the antiviral efficacy of nelfinavir in HIV-1 infected monocytic cells" Gerlach et al. Peptide Science, 2013

• "Insulin-like growth factor binding protein-3 inhibits monocyte adhesion to retinal endothelial cells in high glucose conditions" Zhang et al. Molecular Vision, 2013

• "Synthesis and biological activity of NOSH-naproxen (AVT-219) and NOSH-sulindac (AVT-18A) as potent anti-inflammatory agents with chemotherapeutic potential"  Kodela et al. Medchemcomm, 2013

• "Rk1, a ginsenoside, is a new blocker of vascular leakage acting through actin structure remodeling" Maeng et al. PLoS One, 2013

• "Soluble amyloid-β protein aggregates induce nuclear factor-κB mediated upregulation of adhesion molecule expression to stimulate brain endothelium for monocyte adhesion" Gonzalez-Velasquez et al. J Adhesion Science & Technology, 2012

• "Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells" Kusuhara et al. PLoS One, 2012

• "High-glucose-induced endothelial cell injury is inhibited by a peptide derived from human apolipoprotein E"  Bhattacharjee et al. PLoS One, 2012

• "Optical recording reveals novel properties of GSK1016790A-induced vanilloid transient receptor potential channel TRPV4 activity in primary human endothelial cells" Sullivan et al. Molecular Pharmacology, 2012

• "Fatty acid transport protein expression in human brain and potential role in fatty acid transport across human brain microvessel endothelial cells" Mitchell et al. J Neurochemistry, 2011

• "c-jun amino-terminal kinase and mitogen activated protein kinase 1/2 mediate hepatocyte growth factor-induced migration of brain endothelial cells" Rush et al. Experimental Cell Research, 2007

• "Shiga toxin enhances functional tissue factor on human glomerular endothelial cells: implications for the pathophysiology of hemolytic uremic syndrome" Nestoridi et al. J Thrombosis and Hemostasis, 2005

• "β3-adrenergic receptors regulate retinal endothelial cell migration and proliferation" Steinle et al. J Biological Chemistry, 2003

• "Nerve growth factor regulates human choroidal, but not retinal, endothelial cell migration and proliferation" Steinle and Granger, Autonomic Neuroscience, 2003

• "Vascular endothelial growth factor modulates the transendothelial migration of MDA-MB-231 breast cancer cells through regulation of brain microvascular endothelial cell permeability" Lee et al. J Biological Chemistry, 2003

• "Vascular endothelial growth factor modulates neutrophil transendothelial migration via up-regulation of interleukin-8 in human brain microvascular endothelial cells" Lee et al.  J Biological Chemistry, 2002

• "Elevated endothelial microparticles in thrombotic thrombocytopenic purpura: findings from brain and renal microvascular cell culture and patients with active disease" Jiminez et al. British Journal of Haematology, 2001