Passage Reagent PRG-2 (Trypsin-EDTA Solution 1X), 100ml
A part of the Passage Reagent Group™ (PRG).

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Primary Human Glomerular Mesangial Cells (ACBRI 127)


Gain Consistency with our Single Lot Media and Reagents

The Passage Reagent Group™ (PRG) is a matched set of CSC Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1™ (EDTA-dPBS Solution), PRG-2™ (Trypsin/EDTA-dPBS Solution) and PRG-3™(Trypsin Inhibitor-dPBS Solution) The chelating agent EDTA in PRG-1™ prepares for PRG-2™, which contains highly purified trypsin. PRG-3™ inactivates the protease in PRG-2™ and stabilizes the cell membranes.

Cell membranes are materially and cumulatively damaged whenever cells are exposed to serine proteases, physically manipulated, centrifuged, and/or frozen. Use of the PRG greatly minimizes damage and stress to cells during passage or freezing of cell cultures.

PRG2™ contains highly purified trypsin, allowing a substantial reduction (40%) in the amount of enzyme required to detach cells compared to typical commercial trypsin solutions. Intended for sequential use with PRG1™ (EDTA-dPBS Solution) and PRG3™ (Trypsin Inhibitor-dPBS solution).

 

Storage and Handling

Store at -20°C. Once opened, shelf life 30 days at +2 - 8°C

Cell Systems media and reagents are made with WFI, all components are cGMP and ISO Compliant, and are classified "Sterile".


COMPANION PRODUCTS:


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