A nutrient-rich, customizable medium with physiological glucose levels for endothelial and epithelial cell research.
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The fully defined system for reproducible research
Product overview
OptiCulture™ Serum-Free Medium-R (4E3-500-R) is a defined, nutrient-rich formulation that supports healthy growth and normal phenotypes across diverse cell types. By supporting neurons, glial cells, endothelial cells, and pericytes under serum-free conditions, it promotes cellular health, barrier integrity, and functional phenotype expression. With recombinant human growth factors replacing traditional tissue-derived supplements, it ensures a clean, consistent, and fully defined system for reproducible, translationally relevant results.
This medium is particularly suited for:
- CNS and retinal cell cultures.
- Epithelial and endothelial barrier modeling.
- Neurovascular unit reconstruction.
- Organoid systems and co-culture platforms.
- Disease modeling and drug screening.
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Any application requiring well-defined growth conditions
| Key feature | Key benefit |
| Nutrient-rich composition | Provides essential amino acids, lipids, nucleotides, vitamins, and cofactors to sustain healthy, long-lived cultures and support demanding assays. |
| Customizable supplements | Separate supplement vials allow researchers to adjust formulations for specific cell types, co-cultures, and experimental conditions - maximizing flexibility and reproducibility. |
| Physiological glucose levels | Mirrors in vivo glucose to maintain stable transcriptomic profiles, preserve native cell functions, and reduce experimental artifacts. |
| Recombinant human growth factors | Recombinant human growth factors: A curated set of recombinant factors enhances survival, growth, and phenotypic fidelity across multiple cell types:
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OptiCulture Serum-Free Medium-R (4E3-500-R) kit consists of:
- 500ml nutrient-rich, serum-free basal medium.
- Optiboom-R (4OB-500-R) (human recombinant growth factors).
- Other supplements (ITS, Anti-Oxidants, Hydrocortisone).
- 10 ml Attachment Factor (4Z0-201).
Use with Attachment Factor™
For best results, use Cell Systems medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent. See the Attachment Factor™ protocol.
Medium storage
Cell Systems medium is shipped at ambient temperature. Upon arrival, immediately refrigerate the medium and supplements according to the storage instructions on the label. Once the medium has been supplemented with OptiBoom-R, or any component of the medium kit has been opened, the shelf life is 30 days when stored at 2–8 °C.
Request free sample or more information
Interested in OptiCulture™ Medium for your research? Fill out the form below to request a sample, or contact us for more information.
OptiCulture™ Serum-Free Medium is available in three specialized kits, each formulated with a unique set of supplements to match different research needs. Compare the kits side by side in the table below to find the best fit for your experiments.
| Kit | OptiCulture-RH (4E3-500-RH) | OptiCulture-R (4E3-500-R) | OptiCulture-B (4E3-500-B |
| System type | Fully defined, xeno-free | Fully defined, serum-free | Fully defined, serum-free |
| Applications | Cultures of a broad spectrum of cells including endothelial cells and epithelial cells; xeno-free barrier models; neurovascular unit models; organoids and co-cultures; disease modeling and drug screening. | Cultures of a broad spectrum of cells including endothelial cells and epithelial cells; epithelial and endothelial barrier models; neurovascular unit models; organoids and co-cultures; disease modeling and drug screening. | CNS and retinal cultures; BBB and BRB models; neurovascular unit models; organoids and co-cultures; disease modeling and drug screening. |
| Key differentiator | Completely xeno-free system with physiological glucose content for translational and clinically relevant research workflows. | Fully defined, recombinant growth factor–based system supporting multiple cell types with consistent, reproducible performance under serum-free and physiological glucose content conditions. | CNS- and retinal-focused medium providing a physiologically relevant environment for robust neural and barrier models beyond generic media. |
| Growth factor source | Recombinant human | Recombinant human | Proprietary brain-derived supplementation |
| Coating material (included in kit) | Xeno-free coating material |
COMPANION PRODUCTS:
STREAMLINE YOUR RESEARCH TOOLS
Using Cell Systems optimized media and reagent kits can remove variables from your research program while enhancing consistency.