Complete Medium Formulated at Normal Blood Glucose Level Without Serum and With CultureBoost-R™ (4N3-500-R) - Cell Systems
Complete Medium Kit Formulated at Normal Blood Glucose Level Without Serum and With CultureBoost-R™
Kit consists of 500mL Cell Systems Normal Glucose (0.9008 g/L) Medium [No Serum] and 10mL Attachment Factor™ (extracellular matrix reagent for coating flasks) and 10mL CultureBoost-R™. 4N3-500 is not a complete medium. For complete medium, add 5-20% Serum (Defined FBS or equivalent) and human recombinant growth factor (CultureBoost-R (cat #4CB-500-R)). 4N3-500 is Certified and intended for experimental application. Cell Systems media and reagents are sterile, made with WFI and all components are cGMP and ISO Compliant.
Available in one 500 mL container.
Certified for use with more than 100 human and animal cell cultures and cell lines including:
All ACBRI and CSC Primary Cells
Bone Marrow Cells (CFU-F and Erythroid Burst Cultures)
Connective Tissue Cells
Dermal Fibroblast Cells
Endothelial Cells (Aortic, Arterial, Coronary Artery, Vascular and Venous)
Fetal Lung Cells
Embryonic and Adult Stem Cells
Glomerular Mesangial Cells
Immortalized / Tumor-derived Mesenchymal Cells
Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal)
Smooth Muscle Cells
Tumor Neovascular Cells
Characteristics of Medium formulated without Serum
Cell Systems Medium contains no added hormones.
Medium contains no antibiotics; however, Bac-Off®, (Cat #4Z0-643), is the recommended antibiotic for all Cell Systems medium. Bac-Off® is used at or above the 2µg/mL MIC of aerobic gram-positive and gram-negative microorganisms. (See Bac-Off® certificate.)
Medium contains no phenol red. Phenol red in cell culture medium binds to receptors on cell surfaces and causes the cell to respond as though pharmacologic levels of estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) are present in the medium. We exclude this unnecessary additive in order to optimize experimental conditions for researchers.
Cell Systems Medium is shipped at ambient temperature. If the entire unit will not be used within 30 days, activate the medium with the enclosed supplement then aliquot and freeze in smaller units which will be used within 30 days.
Upon receipt, if the full 500mL medium unit will be used within 30 days, immediately refrigerate the medium and supplements. Once the unit is activated, or any component of the medium kit is opened, the shelf life is 30 days at refrigerated (4 - 8°C) temperatures.
Use with Attachment Factor™
For best results, use Cell Systems Medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent. See the Attachment Factor™ protocol.