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Complete Serum-Free Medium Kit With Recombinant RocketFuel™ (SF-4Z0-500-R) - Cell Systems

Regular price $227.00

Complete Serum-Free Medium Kit With Recombinant RocketFuel™

 

Kit consists of 500mL SF-4Z0-500-R Serum-Free Medium, 8mL vial of RocketFuel™ (containing human recombinant growth factors) and 10mL vial of Attachment Factor™ (extracellular matrix reagent for coating flasks).  This becomes a complete medium once activated with the included RocketFuel™ supplement. This yields a chemically defined complete medium which does not require the addition of bovine serum. SF-4Z0-500-R is Certified and intended for experimental application. Cell Systems media and reagents are sterile, made with WFI and all components are cGMP and ISO Compliant.

Available Formats

Available in one 500 mL container.

Appropriate Use

This medium is recommended for use with a stable proliferating culture when Serum-Free conditions are required for experimentation. For thawing and plating of cryopreserved cells, we recommend Media With Serum.

Certified for use with more than 100 human and animal cell cultures and cell lines including:

Human:

  All ACBRI and CSC Primary Cells
  Bone Marrow Cells (CFU-F and Erythroid Burst Cultures)
  Connective Tissue Cells
  Endothelial Cells ( Aortic, Arterial, Coronary Artery, Vascular and Venous)
  Fetal Lung Cells
  Embryonic and Adult Stem Cells
  Fibroblast Cells
  Glomerular Mesangial Cells
  Immortalized / Tumor-derived Mesenchymal Cells
  Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal)
  Smooth Muscle Cells
  Tumor Neovascular Cells

Animal:
  Bovine and Porcine large vessel and microvessel Endothelial Cells

Characteristics of Cell Systems Serum-Free Medium:

Medium contains no added hormones.

Medium contains no antibiotics; however, Bac-Off® (Cat. #4Z0-643), is the recommended antibiotic for all Cell Systems media. Bac-Off® is used at or above the 2µg/mL MIC of aerobic Gram-positive and Gram-negative microorganisms.

Addition of Bovine Serum is not necessary. Cell Systems Serum-Free Medium is a chemically defined, complete medium which does not contain bovine-derived components. Human serum albumin and other components of human origin are specified by the formulation.

Use with Attachment Factor™

For best results, use Cell Systems Medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent. See the Attachment Factor™ protocol.