A nutrient-rich, xeno-free formulation supporting reproducible growth and function across neural, vascular, and epithelial models.
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- PRODUCT INFO
Start Your Next Translational Model with Fully Defined, Xeno-Free Medium.
Product Overview
OptiCulture™ Serum-Free Medium-RH (4E3-500-RH) eliminates all animal-derived components, making it ideal for researchers focused on translational relevance, clinical compatibility, and reproducibility. Designed to support a broad spectrum of cell types, it provides a nutrient-rich, serum-free environment that promotes cellular health, functional phenotype expression, and barrier integrity - all without reliance on animal-derived biomaterials.
This medium is particularly suited for:
- CNS and retinal cell cultures
- Xeno-free epithelial and endothelial barrier modeling
- Neurovascular unit reconstruction
- Organoid systems and co-culture platforms
- Disease modeling and drug screening
- Any application requiring animal-free, well-defined growth condition.
| Key Feature | Key Benefit |
| Xeno-Free | Formulated without animal-derived components to ensure high reproducibility and bio-/immune-compatibility in sensitive research models. |
| Nutrient-Rich Composition | Provides essential amino acids, lipids, nucleotides, vitamins, and cofactors to sustain healthy, long-lived cultures and support demanding assays. |
| Customizable Supplements | Separate supplement vials allow researchers to adjust formulations for specific cell types, co-cultures, and experimental conditions - maximizing flexibility and reproducibility. |
| Physiological Glucose Levels | Mirrors in vivo glucose to maintain stable transcriptomic profiles, preserve native cell functions, and reduce experimental artifacts. |
| Recombinant human growth factors | Recombinant human growth factors: A curated set of recombinant factors enhances survival, growth, and phenotypic fidelity across multiple cell types:
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OptiCulture Serum-Free Medium-R (4E3-500-R) Kit Consists of:
- 500ml Nutrient-Rich, Serum-Free Basal Medium
- Optiboom-R (4OB-500-R) (human recombinant growth factors)
- Supplements (1 x ITS, 1 x Anti-Oxidants, 1 x Hydrocortisone)
- 10 ml Xeno-Free Coating Material.
Medium Storage
Cell Systems Medium is shipped at ambient temperature. Upon arrival, immediately refrigerate the medium and supplements according to the storage instructions on the label. Once the medium has been supplemented with OptiBoom-R, or any component of the medium kit has been opened, the shelf life is 30 days when stored at 2–8 °C.
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Interested in OptiCulture™ Medium for your research? Fill out the form below to request a sample, or contact us for more information.
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Using Cell Systems optimized media and reagent kits can remove variables from your research program while enhancing consistency.