Engineered for Reliable, Biocompatible Research
OPTICULTURE™
SERUM-FREE MEDIUM
The new standard for precision in advanced human cell models
Nutrient-rich serum-free composition
Physiological glucose levels
Customizable supplements
Phenol red–free
Serum-Free Medium for Advanced Cell-Based Models – Enabling Reliable, Human-Relevant Results
OptiCulture™ is a nutrient-rich, phenol red–free formulation featuring physiological glucose levels and customizable supplements. Whether you are scaling up or fine-tuning complex models, OptiCulture™ delivers consistent, translationally meaningful results.
One medium, many models:
This medium is particularly suited for ex vivo cultures of endothelial, epithelial, CNS and retinal cells for:
- Disease modeling, such as Liver-on-A-Chip, Kidney-on-A-Chip, and Lung-on-A-Chip.
- Drug screening.
- Barrier modeling, such as Blood-Brain Barrier (BBB), Blood-Retinal Barrier (BRB).
Limited Availability - Request Your Free Sample
We’re offering a limited number of 50 mL samples of OptiCulture™ Serum-Free Medium. Fill out the form now to claim yours!
Key Features & Benefits:
- Xeno-free kit OptiCulture™ Serum-Free Medium-RH: Formulated without animal-derived components to ensure high reproducibility and bio-/immune-compatibility in sensitive research models.
- Nutrient-rich serum-free composition. Provides essential amino acids, lipids, nucleotides, vitamins, and cofactors to sustain healthy, long-lived cultures and support demanding assays.
- Physiological glucose levels. Mirrors in vivo glucose to maintain stable transcriptomic profiles, preserve native cell functions, and reduce experimental artifacts.
- Phenol red–free: Designed for cell culture applications requiring minimal background interference, enabling clearer imaging, precise assay readouts, and reliable hormone-sensitive studies.
- Recombinant human growth factors or proprietary brain-derived supplementation. OptiCulture™ kits are available with either recombinant human growth factors (OptiBoom-R™) - broadening support across multiple human cell types - or a proprietary brain-derived supplement (OptiBoom-B™), tailored for CNS, retinal, and neurovascular models.
- Customizable supplements. Separate supplement vials allow researchers to adjust formulations for specific cell types, co-cultures, and experimental conditions - maximizing flexibility and reproducibility.
- Xeno-Free Kit OptiCulture™ Serum-Free Medium-RH: Formulated without animal-derived components to ensure high reproducibility and bio-/immune-compatibility in sensitive research models.
- Nutrient-Rich Serum-Free Composition. Provides essential amino acids, lipids, nucleotides, vitamins, and cofactors to sustain healthy, long-lived cultures and support demanding assays.
- Physiological Glucose Levels. Mirrors in vivo glucose to maintain stable transcriptomic profiles, preserve native cell functions, and reduce experimental artifacts.
- Phenol Red–Free: Designed for cell culture applications requiring minimal background interference, enabling clearer imaging, precise assay readouts, and reliable hormone-sensitive studies.
- Recombinant Human Growth Factors or Proprietary Brain-Derived Supplementation. OptiCulture™ kits are available with either recombinant human growth factors (OptiBoom-R™) - broadening support across multiple human cell types - or a proprietary brain-derived supplement (OptiBoom-B™), tailored for CNS, retinal, and neurovascular models.
- Customizable Supplements. Separate supplement vials allow researchers to adjust formulations for specific cell types, co-cultures, and experimental conditions - maximizing flexibility and reproducibility.
Side-by-Side: Summary and Comparison
| Cat# | OptiCulture-RH (4E3-500-RH) | OptiCulture-R (4E3-500-R) | OptiCulture-B (4E3-500-B |
| System Type | Fully defined, xeno-free | Fully defined, serum-free | Fully defined, serum-free |
| Applications | Cultures of a broad spectrum of cells including endothelial cells and epithelial cells; xeno-free barrier models; neurovascular unit models; organoids and co-cultures; disease modeling and drug screening. | Cultures of a broad spectrum of cells including endothelial cells and epithelial cells; epithelial and endothelial barrier models; neurovascular unit models; organoids and co-cultures; disease modeling and drug screening. | CNS and retinal cultures; BBB and BRB models; neurovascular unit models; organoids and co-cultures; disease modeling and drug screening. |
| Key Differentiator | Completely xeno-free system with physiological glucose content for translational and clinically relevant research workflows. | Fully defined, recombinant growth factor–based system supporting multiple cell types with consistent, reproducible performance under serum-free and physiological glucose content conditions. | CNS- and retinal-focused medium providing a physiologically relevant environment for robust neural and barrier models beyond generic media. |
| Growth Factor Source | Recombinant human | Recombinant human | Proprietary brain-derived supplementation |
| Coating Material (Included in Kit) | Xeno-free coating material |
Choose the set of Supplements that Meet Your Needs
Choose the Set of Supplements that
Meet Your Needs
Our OptiBoom-R™ in OptiCulture™ Serum-Free Medium-RH provides xeno-free and robust supplements, which support the growth and development of normal phenotype of broad spectrum of cells, including endothelial cells and epithelial cells.
Our OptiBoom-R™ in OptiCulture™ Serum-Free Medium-R provides defined and robust supplements, which support the growth and development of normal phenotype of broad spectrum of cells, including endothelial cells and epithelial cells.
Our OptiBoom-B™ in OptiCulture™ Serum-Free Medium-B provides rich and robust nutrients, which support the growth and development of ex vivo phenotype of cells, especially central nervous systems- and retinal-derived endothelial cells, neurons and glial cells.
Request Your Free Sample Today
Interested in OptiCulture™ Medium for your research? Fill out the form below to request a sample, or contact us for more information.
Build Your Complete, Translationally Relevant Research Model Today!
Pair the OptiCulture Serum-Free Medium with validated Cell Systems’ Primary Human Cells.