Cell Systems⎢Cell Freezing Medium
Cell Freezing Medium provides a beneficial environment for the freeze/thaw cycle of cell cultures
Cell Systems Cell Freezing Medium is a specialized medium. When used in conjunction with Cell Systems Passage Reagent Group™, it provides a beneficial environment for the freeze/thaw cycle of cell cultures and assists in minimizing cellular damage during the process.
Cell Systems Cell Freezing Medium (1X) 50 ml. Contains Conditioned Cell Systems Media.
Use of the Passage Reagent Group™ (PRG) to release cells from culture for freezing is critically important in minimizing membrane and cytoskeletal damage and should be used with Cell Systems Cell Freezing Medium. Cell membranes are materially and cumulatively damaged by detachment or when subjected to the stress of freezing and thawing. Use of PRG in conjunction with Cell Freezing Medium and Attachment Factor™ minimizes cellular stress and damage associated with these processes and results in a much better yield.
1. Follow steps # 1 - #12 in the instructions for 4Z0-800 Passage Reagent Group™ (PRG).
2. Resuspend cells in ice-cold Cell Freezing Medium at the desired final cell concentration: the “sweet spot” is typically between 0.5 - 2.0 X 106 cells/mL.
3. Transfer to an ice-cold sterile cryogenic vial. Cell Systems uses CORNING® vials.
4. Reduce the temperature at 1°C per minute until < -70°C is reached.
5. Store cell vials under liquid nitrogen. Temperature fluctuations in nitrogen vapor are bad for cell viability.
Thawing cells (recovery from frozen storage):
Follow the thawing directions appropriate for your medium system, remembering the importance of the correct use of Attachment Factor™.
Handling and Storage
Store refrigerated ~4°C. Once opened, refrigerated shelf life is 30 days.
Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.
- "The Contribution Of Toll-Like Receptors In The Pathogenesis Of Diabetic Retinopathy In Human Microvascular Retinal Endothelial Cells In Vitro" (F Dad Bakhsh, 2016)
- "Soluble aggregates of the amyloid-beta peptide are trapped by serum albumin to enhance amyloid-bets activation of endothelial cells" (Melissa A Moss, 2009)