Passage Reagent Group™ (4Z0-800) - Cell Systems
Passage Reagent Group™ is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing.
This PRG Group™ is qualified for use with all Cell Systems Certified Mediums. The PRG contains three parts: PRG-1™ (EDTA-dPBS Solution), PRG-2™ (Trypsin/EDTA-dPBS Solution) and PRG-3™(Trypsin Inhibitor-dPBS Solution) each in a 100mL bottle. Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.
Cell membranes are materially and cumulatively damaged whenever cells are exposed to serine proteases, physically manipulated, centrifuged, and/or frozen. Use of the PRG greatly minimizes damage and stress to cells during passage or freezing of cell cultures.
PRG-1™ (EDTA-dPBS Solution): The chelating agent EDTA in PRG-1™ prepares the cells for PRG-2™ processing.
PRG-2™: Contains highly purified trypsin, allowing a substantial reduction (40%) in the amount of enzyme required to detach cells compared to typical commercial trypsin solutions. The trypsin in PRG-2™ is inactivated by trypsin inhibitors in the PRG-3™ formulation, preventing nonspecific protease damage to cell membranes after detachment.
PRG-3™: Inactivates the protease in PRG-2™ and stabilizes the cell membranes for passaging and freezing. PRG-3™ is engineered to work together with Attachment Factor™ (Cat. #4Z0-210) to stabilize the cell membrane and promote spreading and the establishment of correct polarity after subculture.
Our Passage Reagent System™ (cat. #4Z0-840), adds AttachmentFactor™ to the PRG Group™.
Storage and Handling
Store at -20°C. Once opened, shelf life 30 days at +4 - 8°C
Cell Systems media and reagents are made with WFI, all components are cGMP and ISO Compliant, and are classed "Sterile".
InstructionsClick to view the Passage Reagent Group Protocol
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