Passage Reagent Group™ is a matched set of Cell Systems Certified™ reagents (3x100mL) for releasing cells from culture for subculture or freezing.

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Primary Human Glomerular Mesangial Cells (ACBRI 127)


Gain Consistency with our Single Lot Media and Reagents

This PRG Group™ is qualified for use with all Cell Systems Certified Mediums. The PRG contains three parts: PRG-1™ (EDTA-dPBS Solution), PRG-2™ (Trypsin/EDTA-dPBS Solution) and PRG-3™(Trypsin Inhibitor-dPBS Solution) each in a 100mL bottle.

Cell membranes are materially and cumulatively damaged whenever cells are exposed to serine proteases, physically manipulated, centrifuged, and/or frozen. Use of the PRG greatly minimizes damage and stress to cells during passage or freezing of cell cultures.

PRG-1™ (EDTA-dPBS Solution): The chelating agent EDTA in PRG-1™ prepares the cells for PRG-2™ processing.

PRG-2™: Contains highly purified trypsin, allowing a substantial reduction (40%) in the amount of enzyme required to detach cells compared to typical commercial trypsin solutions. The trypsin in PRG-2™ is inactivated by trypsin inhibitors in the PRG-3™ formulation, preventing nonspecific protease damage to cell membranes after detachment.

PRG-3™: Inactivates the protease in PRG-2™ and stabilizes the cell membranes for passaging and freezing. PRG-3™ is engineered to work together with Attachment Factor™ (4Z0-210) to stabilize the cell membrane and promote spreading and the establishment of correct polarity after subculture.

Our Passage Reagent System™ (4Z0-840), adds AttachmentFactor™ to the PRG Group™.

 

Storage and Handling

Store at -20°C. Once opened, shelf life 30 days at +2 - 8°C

Cell Systems media and reagents are made with WFI, all components are cGMP and ISO Compliant, and are classified "Sterile".


A Selection of Citations for 4Z0-800 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems reagents.

 


COMPANION PRODUCTS:


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Using Cell Systems optimized media and reagent kits can remove variables from your research program while enhancing consistency.