This complete medium is formulated with 10% serum. Kit consists of 500mL 4N0-500 Cell Systems Normal Glucose Medium, 10mL CultureBoost-R™ and 10mL Attachment Factor™.

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Primary Human Glomerular Mesangial Cells (ACBRI 127)

Gain Consistency with our Single Lot Media and Reagents

Medium is formulated with 10% serum. Kit consists of 500mL 4N0-500-R Cell Systems normal glucose medium (glucose concentration = 0.9008 g/L or 5mM), 10mL CultureBoost-R™ (containing human recombinant growth factors) and 10mL Attachment Factor™ (extracellular matrix reagent for coating flasks). This growth medium has been formulated at normal human plasma glucose level and becomes a complete medium once activated with the included CultureBoost-R™ supplement. 4N0-500-R is Certified and intended for experimental application.


Available Formats

Available in one 500 mL container with two 10mL vials.


Characteristics of Cell Systems Serum-Containing Medium:

Medium contains no added hormones. Medium contains no antibiotics; however, Bac-Off® (4Z0-643), is the recommended antibiotic for all Cell Systems media. Bac-Off® is used at or above the 2µg/mL MIC of aerobic Gram-positive and Gram-negative microorganisms.

Medium contains no phenol red. Phenol red in cell culture medium binds to receptors on cell surfaces and causes the cell to respond as though pharmacologic levels of estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) are present in the medium. We exclude this unnecessary additive in order to optimize experimental conditions for researchers.


Use with Attachment Factor™

For best results, use Cell Systems Medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent. See the Attachment Factor™ protocol.


Medium Storage

Cell Systems Medium is shipped at ambient temperature. If the entire unit will not be used within 30 days, supplement the medium with CultureBoost™, then aliquot and freeze in smaller units which will be used within 30 days. Otherwise, immediately refrigerate the medium and supplement. Once the unit is supplemented, or any component of the medium kit is opened, the shelf life is 30 days at refrigerated (2 - 8°C) temperatures.

4N0-500-R is Certified and intended for experimental application. Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.

A Selection of Citations for 4N0-500-R from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems Median and Reagents.



Appropriate Use

Certified for use with more than 100 human and animal cell cultures and cell lines including:



All ACBRI and CSC Primary Cells

Bone Marrow Cells (CFU-F and Erythroid Burst Cultures)
Connective Tissue Cells
Dermal Fibroblast Cells
Endothelial Cells (Aortic, Arterial, Coronary Artery, Vascular and Venous)
Fetal Lung Cells
Embryonic and Adult Stem Cells
Fibroblast Cells
Glomerular Mesangial Cells
Immortalized / Tumor-derived Mesenchymal Cells
Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal)
Smooth Muscle Cells
Tumor Neovascular Cells


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Using Cell Systems optimized media and reagent kits can remove variables from your research program while enhancing consistency.