CultureBoost™ (4CB-500)

CultureBoost™ Growth Factor for Cell Cultures

This 10 mL Vial supplements 500mL Cell Systems Cell Culture Medium.

CultureBoost™ is the broad-spectrum mitogen in Cell Systems Complete Medium (4Z0-500) and Cell Systems Medium Without Serum (4Z3-500). CultureBoost contains Cell Systems bovine growth factor and porcine heparin. 

CultureBoost™ is purified from bovine pituitary or hypothalamus and is 100% traceable to cattle processed in the USA at USDA inspected facilities. The product's working concentration is 50µg/mL. Biological activity is adjusted to 110% of maximum using CSC 2V0 Human Venous Endothelial cells,  ACBRI 181 Primary Retinal Microvascular Endothelial cells, and ACBRI 376 Primary Brain Microvascular Endothelial cells.

A 10mL vial is included in various Cell Systems media kits and in "The System™". CultureBoost™ 10mL vials are also available individually here. 

Use

CultureBoost™ has two main uses.

1.  Application as a growth supplement to medium:

Cell Systems Complete Medium Kit (cat. #4Z0-500) and Cell Systems Medium Minus Serum Kit (cat. #4Z3-500) each include a 5mL vial of CultureBoost™ (cat. #4CB-500-R).

CultureBoost™ can be purchased separately (cat. #4CB-500) to supplement Cell Systems Media Kit without Growth Factor (cat. # 4Z0-500-S) or other cell culture media.

 2. Application in the vial thaw procedure:

Cells are at their most fragile when in recovery from thawing or passage. The growth factors in CultureBoost™ and CultureBoost-R™ stabilize the plasma membrane and provide a concentrated dose of factors that have been shown to aid the cell in attachment, activation, and achievement of polarity.

CultureBoost™ can be beneficially used any time cells are "loosened" from the pellet prior to resuspension in Cell Systems Medium in preparation for plating. The best results of using CultureBoost™ are evident when 50-100 microliters are added to the loosened cell pellet just prior to re-suspension in medium. Please refer to the instructions for our Passage Reagent Group™ (cat. #4Z0-800).

Handling and Storage

This product is delivered at ambient temperature. Use 10ml to supplement 500ml cell culture medium. If the entire unit will not be used within 30 days, aliquot and freeze. Once opened, shelf life is 30 days at +2-8°C.

Select Citations

• "Improvement in diabetic retinopathy through protection against retinal apoptosis in spontaneously diabetic Torii rats mediated by ethanol extract of Osteomeles schwerinae C.K. Schneid" Kim, Kim, Kim, et al. Nutrients, 2019.

• "SiRNA silencing of VEGF, IGFs, and their receptors in human retinal microvascular endothelial cells" Nicolau et al American J Translational Research, 2018
• "Human retinal endothelial cells and astrocytes cultured on 3-D scaffolds for ocular drug discovery and development" Beharry et al. Prostaglandins and Other Lipid Mediators, 2018

• "Tangential flow filtration for highly efficient concentration of extracellular vesicles from large volumes of fluid" Busatto and Vilanilam et al. Cells, 2018.

• "Barrier properties and transcriptome expression in human iPSC‐derived models of the blood–brain barrier"  Delsing et al. Stem Cells, 2018.

• "MnTBAP or catalase Is more protective against oxidative stress in human retinal endothelial cells exposed to Intermittent hypoxia than their co-administration" Quan et al. Reactive Oxygen Species, 2017

• "Association of HMGB1 with oxidative stress markers and regulators in PDR" Abu El-Asrar and Alam et al. Molecular Vision, 2017.

• "Neuropilin-1 modulates interferon-γ-stimulated signaling in brain microvascular endothelial cells" Wang et al. Journal of Cell Science, 2016.

• "Fenofibrate Inhibits cytochrome P450 epoxygenase 2C activity to suppress pathological ocular angiogenesis" Gong et al. EBioMedicine, 2016.

• "Primary human liver co-culture With flow and Kupffer cell integration on microfluidic liver-on-a-chip" Mazur  (Dissertation thesis), 2015.

• "Heregulin-HER3-HER2 signaling promotes matrix metalloproteinase-dependent blood-brain-barrier transendothelial migration of human breast cancer cell lines" Momeny et al. Oncotarget, 2015.

• "Aflibercept traps galectin-1, an angiogenic factor associated with diabetic retinopathy"  Kanda et al.  Scientific Reports, 2015.

• "Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells"  Liu et al. PLoS One, 2014.

• "S100A4 is upregulated in proliferative diabetic retinopathy and correlates with markers of angiogenesis and fibrogenesis" Abu El-Asrar et al. Molecular Vision, 2014.

• "Differential expression and role of hyperglycemia induced oxidative stress in epigenetic regulation of β1, β2 and β3-adrenergic receptors  in retinal endothelial cells" Safi et al. BMC Medical Genomics, 2014.

• "Optical recording reveals novel properties of GSK1016790A-induced vanilloid transient receptor potential channel TRPV4 activity in primary human endothelial cells" Sullivan et al. Molecular Pharmacology, 2012.

• "High-glucose-induced endothelial cell injury is inhibited by a peptide derived from human Apolipoprotein E"  Bhattacharjee et al. PLoS One, 2012.