CultureBoost™ (4CB-500) - Cell Systems
CultureBoost™ Growth Factor for Cell Cultures
This 10 mL (50x) Vial activates 500mL Cell Systems Cell Culture Medium.
CultureBoost™ is the broad-spectrum mitogen in Cell Systems Complete Medium (4Z0-500) and Cell Systems Medium Without Serum (4Z3-500). CultureBoost contains Cell Systems bovine growth factor and porcine heparin.
CultureBoost™ is purified from bovine pituitary or hypothalamus and is 100% traceable to cattle processed in the USA at USDA inspected facilities. The product's working concentration is 50µg/mL. Biological activity is adjusted to 110% of maximum using CSC 2V0 Human Venous Endothelial cells, ACBRI 181 Primary Retinal Microvascular Endothelial cells, and ACBRI 376 Primary Brain Microvascular Endothelial cells.
CultureBoost™ has two main uses.
1. Application as a growth supplement to medium:
- Cell Systems Complete Medium Kit (cat. #4Z0-500) includes a 10mL vial of CultureBoost™.
- Cell Systems Medium Kit Without Serum (cat. #4Z3-500) includes a 10mL vial of CultureBoost™.
- Cell Systems Medium Kit Formulated Without Growth Factor (cat. #4Z0-500-S) differs from complete medium only by the absence of growth factors and can be activated and made complete by the addition of CultureBoost™ (cat. #4CB-500).
- Cell Systems Medium Kit Formulated Without Serum and Without Growth Factors (cat. #4Z3-500-S) differs from complete medium by the absence of serum and growth factors and can be made complete by the addition of 10-20% of serum, and CultureBoost (cat. #4CB-500).
2. Application in the vial thaw procedure:
- Cells are at their most fragile when in recovery from thawing or passage. CultureBoost™ stabilizes the plasma membrane and provides a concentrated dose of factors that have been shown to aid the cell in attachment, activation, and achievement of polarity.
- CultureBoost™ can be beneficially used any time cells are "loosened" from the pellet prior to resuspension in Cell Systems Medium in preparation for plating. The best results from using CultureBoost™ are evident when 50-100 microliters (one drop) are added to the loosened cell pellet just prior to re-suspension in media. Please refer to the instructions for Cell Systems Passage Reagent Group™ (cat. #4Z0-800). CultureBoost™ should be added following step 10.
Handling and Storage
This product is delivered at ambient temperature, is filter-sterilized and isotonic. If the entire unit will not be used within 30 days, aliquot and freeze. Shelf life is 30 days at +4°C. Do not warm product before use, as the relevant protocols specify that the cells be at ice-water temperature.
- "Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid" Busatto and Vilanilam et al.Cells, 2018
- "Barrier properties and transcriptome expression in human iPSC‐derived models of the blood–brain barrier" Delsing et al. Stem Cells, 2018
- "Association of HMGB1 with oxidative stress markers and regulators in PDR" Abu El-Asrar and Alam et al. Molecular Vision, 2017
- "Fenofibrate Inhibits Cytochrome P450 Epoxygenase 2C Activity to Suppress Pathological Ocular Angiogenesis" Gong et al. EBioMedicine, 2016
- "Primary Human Liver Co-Culture With Flow and Kupffer Cell Integration On Microfluidic Liver-On-A-Chip" Mazur (Dissertation thesis), 2015
- "Heregulin-HER3-HER2 signaling promotes matrix metalloproteinase-dependent blood-brain-barrier transendothelial migration of human breast cancer cell lines" Momeny et al. Oncotarget, 2015
- "Aflibercept Traps Galectin-1, an Angiogenic Factor Associated with Diabetic Retinopathy" Kanda et al. Scientific Reports, 2015
- "Chemokine Receptor CXCR7 Is a Functional Receptor for CXCL12 in Brain Endothelial Cells" Liu et al. PLoS One, 2014
- "S100A4 is upregulated in proliferative diabetic retinopathy and correlates with markers of angiogenesis and fibrogenesis" Abu El-Asrar et al. Molecular Vision, 2014
- "Differential expression and role of hyperglycemia induced oxidative stress in epigenetic regulation of β1, β2 and β3-adrenergic receptors in retinal endothelial cells" Safi et al. BMC Medical Genomics, 2014
- "Optical Recording Reveals Novel Properties of GSK1016790A-Induced Vanilloid Transient Receptor Potential Channel TRPV4 Activity in Primary Human Endothelial Cells" Sullivan et al. Molecular Pharmacology, 2012
- "High-Glucose-Induced Endothelial Cell Injury Is Inhibited by a Peptide Derived from Human Apolipoprotein E" Bhattacharjee et al. PLoS One, 2012