Medium Kit Without Serum and With CultureBoost™ (4Z3-500) - Cell Systems
Medium Kit Without Serum and With CultureBoost™
Kit consists of 500mL 4Z3-500 Medium, 10mL CultureBoost™ (containing animal derived growth factors) and 10mL Attachment Factor™ (extracellular matrix reagent for coating flasks). This is not a complete medium. For complete medium, add 5-20% Serum (Defined FBS or equivalent) and the included CultureBoost™ supplement. 4Z3-500 is Certified and intended for experimental application. Cell Systems mediums and reagents are sterile, made with WFI and all components are cGMP and ISO Compliant.
Available in one 500 mL container.
Certified for use with more than 100 human and animal cell cultures and cell lines including:
All ACBRI and CSC Primary Cells
Bone Marrow Cells (CFU-F and Erythroid Burst Cultures)
Connective Tissue Cells
Dermal Fibroblast Cells
Endothelial Cells (Aortic, Arterial, Coronary Artery, Vascular and Venous)
Fetal Lung Cells
Embryonic and Adult Stem Cells
Glomerular Mesangial Cells
Immortalized / Tumor-derived Mesenchymal Cells
Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal)
Smooth Muscle Cells
Tumor Neovascular Cells
Bovine and Porcine large vessel and microvessel Endothelial Cells
Characteristics of Medium formulated without Serum:
Medium contains no added hormones.
Medium contains no antibiotics; however, Bac-Off®, (Cat #4Z0-643), is the recommended antibiotic for all Cell Systems medium. Bac-Off® is at or above the [2ng/mL] MIC of aerobic gram-positive and gram-negative microorganisms. (See Bac-Off® certificate.)
Medium contains no phenol red. Phenol red in cell culture medium binds to receptors on cell surfaces and causes the cell to respond as though pharmacologic levels of estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) are present in the medium. We exclude this unnecessary additive in order to optimize experimental conditions for researchers.
Medium Storage: If the entire unit will not be used within 30 days, supplement the medium with serum (not included) and CultureBoost™, then aliquot and freeze in smaller units which will be used within 30 days. Otherwise, immediately refrigerate the medium and supplement. Once the unit is supplemented, or any component of the medium kit is opened, the shelf life is 30 days at refrigerated (4-8°C) temperatures.
Use with Attachment Factor™
For best results, use Cell Systems Medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent. See the Attachment Factor™ protocol.
- "High Circulatory Phosphate Level Is Associated with Cerebral Small-Vessel Diseases" Chung et al. Translational Stroke Research, 2018
- "α-Klotho expression determines nitric oxide synthesis in response to FGF-23 in human aortic endothelial cells" Chung et al. PLOS One, 2017
- "Brain Endothelial Cells Produce Amyloid beta From Amyloid Precursor Protein 770 and Preferentially Secrete The O-Glycosylated Form" Kitazume et al Journal of Biological Chemistry, 2010.
- "Mitochondrial Effects of Estrogen Are Mediated by Estrogen Receptor alpha in Brain Endothelial CellsMitochondrial Effects of Estrogen Are Mediated by Estrogen Receptor alpha in Brain Endothelial Cells" Razmara et al The Journal of Pharmacology and Experimental Therapeutics, 2008.