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Cell Systems⎢Medium Kit With Serum and Without Growth Factor (4Z0-500-S)

Regular price $163.00

Medium Kit With Serum and Without Growth Factor 

Medium is formulated with 10% serum. Kit consists of 500mL 4Z0-500-S Cell Systems Medium (formulated without growth factor) and 10mL Attachment Factor™. This is not a complete medium. For complete medium, add animal derived growth factor (CultureBoost™, 4CB-500) or human recombinant growth factor (CultureBoost-R™ 4CB-500-R). 4Z0-500-S is Certified and intended for experimental application. Cell Systems media and reagents are sterile, made with WFI and all components are cGMP and ISO Compliant. 

Available Formats

Available in one 500 mL container.

Appropriate Use

Certified for use with more than 100 human and animal cell cultures and cell lines including these ACBRI Certified cells:

Human:
Bone Marrow Cells (CFU-F and Erythroid Burst Cultures)
Connective Tissue Cells
Dermal Fibroblast Cells
Endothelial Cells (Aortic, Arterial, Coronary Artery, Vascular and Venous)
Fetal Lung Cells
Embryonic and Adult Stem Cells
Fibroblast Cells
Glomerular Mesangial Cells
Immortalized / Tumor-derived Mesenchymal Cells
Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal)
Smooth Muscle Cells
Tumor Neovascular Cells

Animal:
Bovine and Porcine large vessel and microvessel Endothelial Cells

Characteristics of Cell Systems Serum-Containing Medium

Cell Systems Medium contains no added hormones.

Cell Systems Medium contains no antibiotics; however, Bac-Off® (Cat. #4Z0-643), is the recommended antibiotic for all Cell Systems medium. Bac-Off® is at or above the [2ng/mL] MIC of aerobic gram-positive and gram-negative microorganisms. (See Bac-Off® certificate.)

Cell Systems Medium contains no phenol red. Phenol red in cell culture medium binds to receptors on cell surfaces and causes the cell to respond as though pharmacologic levels of estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) are present in the medium. It is difficult to understand how a realistic baseline can be ascertained under these conditions. The side-effects of phenol red can be avoided very simply: leave it out. The presence of phenol red is unnecessary and potentially catastrophic to experimentation.

 

Companion Products

Passage Reagent Group
Passage Reagent Group™

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Bac-Off
Bac-Off®

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Cell Freezing Medium
Cell Freezing Medium

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 Citations