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Cell Systems⎢Complete Medium Kit Formulated at Normal Blood Glucose Level Without Serum and Without CultureBoost™ (4N3-500-S)

Regular price $195.00

Complete Medium Kit Formulated at Normal Blood Glucose Level Without Serum and Without CultureBoost™


Kit consists of 500mL Cell Systems Normal Glucose (0.9008 g/L) Medium and 10mL Attachment Factor™. 4N3-500-S is not a complete medium. For complete medium, add 10 - 20% Serum (Defined FBS or equivalent) and animal derived growth factor, CultureBoost™ (cat #4CB-500) or human recombinant growth factor, CultureBoost-R (cat #4CB-500-R). 4N3-500-S is Certified and intended for experimental application. Cell Systems media and reagents are sterile, made with WFI and all components are cGMP and ISO Compliant.

Available Formats

Available in one 500 mL container.

Appropriate Use

Certified for use with more than 100 human and animal cell cultures and cell lines including these ACBRI Certified cells:


Bone Marrow Cells (CFU-F and Erythroid Burst Cultures)
Connective Tissue Cells
Dermal Fibroblast Cells
Endothelial Cells (Aortic, Arterial, Coronary Artery, Vascular and Venous)
Fetal Lung Cells
Embryonic and Adult Stem Cells
Fibroblast Cells
Glomerular Mesangial Cells
Immortalized / Tumor-derived Mesenchymal Cells
Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal)
Smooth Muscle Cells
Tumor Neovascular Cells


Bovine and Porcine large vessel and micro vessel Endothelial Cells

Characteristics of Cell Systems Serum-Containing Medium

Medium contains no added hormones.

Medium contains no antibiotics; however, Bac-Off® (Cat. #4Z0-643), is the recommended antibiotic for all Cell Systems medium. Bac-Off® is at or above the [2ng/mL] MIC of aerobic gram-positive and gram-negative microorganisms. (See Bac-Off® certificate.)

Medium contains no phenol red. Phenol red in cell culture medium binds to receptors on cell surfaces and causes the cell to respond as though pharmacologic levels of estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) are present in the medium. It is difficult to understand how a realistic baseline can be ascertained under these conditions. The side-effects of phenol red can be avoided very simply: leave it out. The presence of phenol red is unnecessary and potentially catastrophic to experimentation.

Medium Storage
Cell Systems Serum containing Medium is shipped at ambient temperature. If the entire unit will not be used within 30 days, activate the medium with the enclosed supplement then aliquot and freeze in smaller units which will be used within 30 days.

If the full 500mL medium unit will be used within 30 days, immediately refrigerate the medium and supplements.  Once the unit is activated, or any component of the medium kit is opened, the shelf life is 30 days at refrigerated (4 - 8°C) temperatures.


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