This complete medium consists of 500mL SF-4Z0-500 Serum-Free Medium, 8mL vial of RocketFuel™, and 10mL vial of Attachment Factor™

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Primary Human Glomerular Mesangial Cells (ACBRI 127)

Gain Consistency with our Single Lot Media and Reagents

Kit consists of 500mL SF-4Z0-500 Serum-Free Medium, 8mL vial of RocketFuel™ (containing animal derived growth factors), and 10mL vial of Attachment Factor™ (extracellular matrix reagent for coating flasks). This becomes a complete medium once activated with the included RocketFuel™ supplement. This yields a chemically defined complete medium which does not require the addition of bovine serum. SF-4Z0-500 is Certified and intended for experimental application.


Available Formats

Available in one 500 mL container with 8mL and 10mL vials.


Characteristics of Cell Systems Serum-Free Medium:

Medium contains no added hormones. Medium contains no antibiotics; however, Bac-Off® (4Z0-643), is the recommended antibiotic for all Cell Systems media. Bac-Off® is used at or above the 2µg/mL MIC of aerobic Gram-positive and Gram-negative microorganisms.

Medium contains no phenol red. Phenol red in cell culture medium binds to receptors on cell surfaces and causes the cell to respond as though pharmacologic levels of estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) are present in the medium. We exclude this unnecessary additive in order to optimize experimental conditions for researchers.


Addition of Bovine Serum is not necessary.

Cell Systems Serum-Free Medium is a chemically defined, complete medium which does not contain bovine-derived components. Please note that RocketFuel™ does contain bovine-derived growth factors. For a bovine-free supplement, see RocketFuel-R™. Human serum albumin and other components of human origin are specified by the formulation.


Use with Attachment Factor™

For best results, use Cell Systems Medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent. See the Attachment Factor™ protocol.


Medium Storage

Cell Systems Medium is shipped at ambient temperature. If the entire unit will not be used within 30 days, supplement the medium with CultureBoost™, then aliquot and freeze in smaller units which will be used within 30 days. Otherwise, immediately refrigerate the medium and supplement. Once the unit is supplemented, or any component of the medium kit is opened, the shelf life is 30 days at refrigerated (2 - 8°C) temperatures.

SF-4Z0-500 is Certified and intended for experimental application. Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.

A Selection of Citations for SF-4Z0-500 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems Media and Reagents.


Appropriate Use

This medium is recommended for use with a stable proliferating culture when Serum-Free conditions are required for experimentation. For thawing and plating of cryopreserved cells, we recommend Media With Serum.

Certified for use with more than 100 human and animal cell cultures and cell lines including:



All ACBRI and CSC Primary Cells

Bone Marrow Cells (CFU-F and Erythroid Burst Cultures)
Connective Tissue Cells
Dermal Fibroblast Cells
Endothelial Cells (Aortic, Arterial, Coronary Artery, Vascular and Venous)
Fetal Lung Cells
Embryonic and Adult Stem Cells
Fibroblast Cells
Glomerular Mesangial Cells
Immortalized / Tumor-derived Mesenchymal Cells
Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal)
Smooth Muscle Cells
Tumor Neovascular Cells



Bovine and Porcine large vessel and microvessel Endothelial Cells


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Using Cell Systems optimized media and reagent kits can remove variables from your research program while enhancing consistency.