These antibody-free human primary cells were isolated by elutriation of dispase dissociated dermal tissue without the use of positive selection of antibodies.
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Increased Biological Relevance from Primary Human Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. Keratinocytes offer a valuable research tool for areas of research including pain and neuropathy, drug testing and toxicity. These keratinocytes are especially pure (99.8%) and with our inventory offer a consistent tool that you can rely on for the duration of your research program. Ask about our large lots and reservations.
These primary cells require specialized media (with low levels of calcium) that is not currently available at Cell Systems. Please inquire at customerservice@cell-systems.com for recommendations. These cells requires use of tissue culture grade flasks, dish or well with no additional coating.
- Isolated from normal, healthy tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Viability is greater than 92% in our lab environment
- The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost-R™.
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Standard Tests
TESTS | RESULTS |
HIV Serologic Test (donor level HIV AB EIA) | Negative |
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
TESTS | RESULTS |
Bacterial Sterility (culture method) by independent lab | Pass |
Fungal Sterility (culture method) by independent lab | Pass |
Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
TESTS | RESULTS |
Cytokeratin 5/14 | 99.8% positive by immunofluorescence |