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Serum-Free Medium Kit Without Growth Factor (SF-4Z0-500-S) - Cell Systems

Regular price $227.00

Serum-Free Medium Kit Without Growth Factor

Kit consists of 500mL SF-4Z0-500-S Serum-Free Medium and 10mL vial of Attachment Factor™ (extracellular matrix reagent for coating flasks). (RocketFuel-S in picture, sold separately). This is not a complete medium. Add serum-free growth factor such as RocketFuel™ or RocketFuel-R to yield a serum-free, chemically defined complete medium. SF-4Z0-500-S is Certified and intended for experimental application. Cell Systems mediums and reagents are sterile, made with WFI and all components are cGMP and ISO Compliant.

Available Formats

Available in one 500 mL container.

Appropriate Use

This medium is recommended for use with a stable proliferating culture when Serum-Free conditions are required for experimentation. For thawing and plating of cryopreserved cells, we recommend Media With Serum.

Certified for use with more than 100 human and animal cell cultures and cell lines including:

Human:
  All ACBRI and CSC Primary Cells

  Bone Marrow Cells (CFU-F and Erythroid Burst Cultures)
  Connective Tissue Cells
  Endothelial Cells ( Aortic, Arterial, Coronary Artery, Vascular and Venous)
  Fetal Lung Cells
  Embryonic and Adult Stem Cells
  Fibroblast Cells
  Glomerular Mesangial Cells
  Immortalized / Tumor-derived Mesenchymal Cells
  Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal)
  Smooth Muscle Cells
  Tumor Neovascular Cells


Animal:
  Bovine and Porcine large vessel and microvessel Endothelial Cells

Characteristics of Cell Systems Serum-Free Medium:

Medium contains no added hormones.

Medium contains no antibiotics; however, Bac-Off® (Ciprofloxacin), Catalog #4Z0-643, is the recommended antibiotic for all Cell Systems Medium.

Addition of Bovine Serum is not necessary. When supplemented with serum-free growth supplement such as RocketFuel-R, Cell Systems Serum-Free Medium is a chemically defined, complete medium which does not contain bovine-derived components. Human serum albumin and other components of human origin are specified by the formulation.

Medium Storage.
If the entire unit will not be used within 30 days, supplement the medium with growth factor such as RocketFuel or RocketFuel-R (each sold separately), then aliquot and freeze in smaller units which will be used within 30 days. Otherwise, immediately refrigerate the medium.  Once the unit is supplemented, or the medium is opened, the shelf life is 30 days at refrigerated (4-8°C) temperatures.

Use with Attachment Factor™

For best results, use Cell Systems Medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent. See the Attachment Factor™ protocol.


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