Research on Diabetic Retinopathy using Cell Systems Primary Cells and Media

Cell Systems primary cells and specialized media are valuable tools to the field of ophthalmology research. Since 2016, our primary cells have been cited in more than 22 publications in ophthalmology-focused journals such as Nature: Eye, Molecular Vision, and IOVS.

The Bartoli Lab, at the Medical College of Georgia, recently published their work investigating microRNA regulation of cell senescence in a model of diabetic retinopathy¹. They relied on our Primary Human Retinal Endothelial Cells cultured in our specialized media (4N0-500), which contains the normal human blood concentration of glucose (~5mM)². To emulate hyperglycemic conditions, the researchers cultured the cells in media with high glucose (25mM). They report that high glucose conditions increase microRNA-34a expression and concomitant cellular senescence. The effect could be prevented with a microRNA antagonist delivered to the Primary Human Retinal Endothelial Cells.

Cell Systems is proud to support such diverse and important research using our life science tools.

References

¹ Thounaojam et al. “MicroRNA-34a (miR-34a) mediates retinal endothelial cell premature senescence through mitochondrial dysfunction and loss of antioxidant activites.” Antioxidants (2019) 8(9):328.

² Danaei and Finucane, et al. “National, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 2·7 million participants”. Lancet (2011) 378:31.