The 5-Second Solution To Efficient Coating of Cell Cultures

Your cell culture coating solution can greatly affect not only cell health and proliferation, but also your lab and protocol schedule and research program velocity.

Cell Systems’ Attachment Factor is your optimized solution to promoting cell attachment for all types of tissue culture surfaces. Unlike other coating solutions (such as collagen) that require you to prepare and coat your tissue culture wares well in advance, Attachment Factor’s coating will be ready for cell plating after just 5 seconds.

Attachment Factor gives you the flexibility to start coating your tissue culture wares when you are truly ready to plate cells. No preparation, no heading into lab early.

This simple coating solution can be easily used in many cell biology applications, including 2D and 3D cell cultures, organs-on-a-chips, microfluidic devices, a wide variety of assays, experiments in which a tight monolayer is crucial, and more.

For example, Attachment Factor-coated transwell inserts were used in a 2020 study by Su et al. to construct an in vitro blood-brain barrier (BBB) monolayer for permeability testing. In this study, the coating solution was also used on glass cover slips for imaging of the cells. Furthermore, Attachment Factor has also been trusted for research on COVID-19 all over the world.

Attachment of other cell types can also be enhanced using Cell Systems’ 5-second coating solution. This includes, but is not limited to, liver sinusoidal endothelial cells, as demonstrated in this 3D Microfluidic Human Liver Chip study on breast cancer metastasis, and retinal endothelial cells in culture dishes for diabetic retinopathy.

Cell Systems Attachment Factor (cat#. 4Z0-201 for 10 mL or 4Z0-210 for 100 mL) is always in stock for same-day shipping. For more publications using Attachment Factor, please check out the product citations here.


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