In Vitro BBB Monolayer Model Explores Permeability of Nanomaterials

Transporting therapeutic drugs and agents across the blood-brain barrier (BBB) has always been a challenge for brain disease therapy. This study examines the permeability of 2D graphene nanosheets through the BBB using a popular in vitro BBB monolayer model constructed with Cell Systems primary human brain microvascular endothelial cells or hBMECs (Cat# ACBRI 376).

The BBB monolayer model is popular due to its cost-effectiveness compared to animal studies and because of the human primary cells’ ability to form tight junctions similar to that observed in the human biological system.

To construct the model, hBMECs (Cat# ACBRI 376) were seeded in cultureware and transwell inserts that were previously coated with our Attachment Factor (Cat# 4Z0-210) to promote cell attachment, correct polarity, and cytoskeletal organization. Complete Classic Medium with Serum (Cat# 4Z0-500), supplemented with CultureBoost growth factor (Cat# 4CB-500), was used to optimize the monolayer.

Similar to hBMECs, brain pericytes cells (Cat# ACBRI 498) also play a critical role in the formation and maintenance of the BBB. To see more studies on how these two cell types are used in BBB research, check out our large collections of citations on their product pages:

Primary human brain microvascular endothelial cells (ACBRI 376)

Primary human brain pericytes (ACBRI 498)

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