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Primary Human Retinal Microvascular Endothelial Cells (ACBRI 181)⎢Cell Systems

Regular price $735.00

Primary Human Retinal Microvascular Endothelial Cells (ACBRI 181) are initiated by elutriation of dispase dissociated normal human retina tissue.

HRMEC (ACBRI 181) 48 hours after plating

Cell Initiation

These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask. In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).

Companion Products

Each vial of cells is shipped with Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.

These cells are qualified for use with:

Complete Classic Medium Kit with Serum and CultureBoost™
Complete Classic Medium Kit with Serum and CultureBoost™

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Complete Medium Normal Glucose Formulation
Complete Medium Kit Formulated at Normal Blood Glucose Level

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The System | All-in-One Kit
The System™: The All-In-One Cell Growth and Passage System

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Standard Tests

      TEST RESULTS
      HIV Serologic Test (donor level HIV AB EIA) Negative
      HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab) Negative
      Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level)
      Negative
      Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) Negative

      Miscellaneous Tests

      TEST RESULTS
      Cytoplasmic VWF / Factor VIII  > 95% positive by immunofluorescence
      Cytoplasmic uptake of Di-I-Ac-LDL  > 95% positive by immunofluorescence
      Cytoplasmic CD31
      > 95% positive by immunofluorescence
      GFAP (Glial Fibrillary Acidic Protein)
      < 1% by immunofluorescence
      Glutamine Synthetase 
      < 1% by immunofluorescence
      NG2 (Neural/Glial Antigen 2)
      < 1% by immunofluorescence
      PDGFR-beta (Platelet Derived Growth Factor Receptor beta)
      < 1% by immunofluorescence

      Citations


          Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization. 

          Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.

          Please read our Terms and Conditions