These antibody-free human primary cells were isolated by elutriation of dispase dissociated normal human retinal tissue without the use of positive selection of antibodies.
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Increase Biological Relevance with Human Primary Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Isolated from normal, healthy donor tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.
A Selection of Citations for ACBRI 181 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.
- "Erianin Controls Collagen-Mediated Retinal Angiogenesis via the RhoA/ROCK1 Signaling Pathway Induced by the alpha2/beta1 Integrin-Collagen Interaction" Li and Liu et al. IOVS, 2022.
- "PKA and Epac1 Reduce Nek7 to Block the NLRP3 Inflammasome Proteins in the Retinal Vasculature" Liu et al. IOVS, 2022.
- "Axl Is Essential for in-vitro Angiogenesis Induced by Vitreous From Patients With Proliferative Diabetic Retinopath" Wu et al. Frontiers in Medicine, 2021.
- "Dihydroartemisinin ameliorates retinal vascular dysfunction in diabetes mellitus via FASN/Kmal-mTOR/SREBP1 feedback loop" Gu et al. Pharmacological Research, 2021.
- "Dose Response of Bumetanide on Aquaporins and Angiogenesis Biomarkers in Human Retinal Endothelial Cells Exposed to Intermittent Hypoxia" Guzel et al. Pharmaceuticals, 2021.
- "RAGE silencing deters CML-AGE induced inflammation and TLR4 expression in endothelial cells" Ramya et al. Experimental Eye Research, 2021.
- "A small molecular multi-targeting tyrosine kinase inhibitor, anlotinib, inhibits pathological ocular neovascularization" Lu et al. Biomedicine & Pharmacotherapy, 2021.
- “The long non-coding RNA HOTAIR is a critical epigenetic mediator of angiogenesis in diabetic retinopathy" Biswas et al. IOVS, 2021.
- "Discovery of novel small-molecule antiangiogenesis agents to treat diabetic retinopathy" Kim and Choi et al. Journal of Medicinal Chemistry, 2021.
- "Dysregulation of miR-374a is involved in the progression of diabetic retinopathy and regulates the proliferation and migration of retinal microvascular endothelial cells" Wang et al. Clinical and Experimental Optometry, 2021.
- "Epac1 regulates TLR4 signaling in the diabetic retinal vasculature" Liu et al. Cytokine, 2021.
- "Regulation of Rac1 transcription by histone and DNA methylation in diabetic retinopathy" Kowluru et al. Scientific Reports, 2021.
- "CD146/Soluble CD146 Pathway Is a Novel Biomarker of Angiogenesis and Inflammation in Proliferative Diabetic Retinopathy" Ahmed M. Abu El-Asrar et al. IOVS, 2021.
- "Nuclear factor of activated T-cells (NFAT) regulation of IL-1β-induced retinal vascular inflammation" Giblin et al. BBA Molecular Basis of Disease, 2021.
- "Hyperoxia Inhibits Proliferation of Retinal Endothelial Cells in a Myc-Dependent Manner" Singh et al. Life, 2021.
- "Breakfast partly restores the anti-inflammatory function of high-density lipoproteins from patients with type 2 diabetes mellitus" Lemmers et al. Atherosclerosis Plus, 2021.
- "A Fully Human Monoclonal Antibody Targeting cKIT Is a Potent Inhibitor of Pathological Choroidal Neovascularization in Mice" Seo et al. Pharmaceutics, 2021.
- "COL10A1 is a novel factor in the development of choroidal neovascularization" Lv et al. Microvascular Research, 2021.
- "Forskolin regulates retinal endothelial cell permeability through TLR4 actions in vitro" Liu et al. Molecular and Cellular Biochemistry, 2021.
- "Interleukin-6 Trans-Signaling Mediated Regulation of Paracellular Permeability in Human Retinal Endothelial Cells" Glass et al. International Journal of Translational Medicine, 2021.
- "The Role of the Wnt Pathway in VEGF/Anti-VEGF-Dependent Control of the Endothelial Cell Barrier" Li et al. IOVS, 2021.
- "CD40 Expressed in Endothelial Cells Promotes Upregulation of ICAM-1 But Not Pro-Inflammatory Cytokines, NOS2 and P2X7 in the Diabetic Retina" Yu et al. IOVS, 2021.
- "Preparation and application of 0D-2D nanomaterial hybridheterostructures for energy applications" Sinha et al. Materials Today Advances, 2021.
- "Knockdown of Long Non-coding RNA TUG1 Suppresses Migration and Tube Formation in High Glucose-Stimulated Human Retinal Microvascular Endothelial Cells by Sponging miRNA-145" Shi et al. Molecular Biotechnology, 2021.
- "miR-139-5p promotes neovascularization in diabetic retinopathy by regulating the phosphatase and tensin homolog" Zhang et al. Archives of Pharmacal Research, 2021.
- "Melatonin exerts protective effects on diabetic retinopathy via inhibition of Wnt/β-catenin pathway as revealed by quantitative proteomics" Yan et al. Experimental Eye Research, 2021.
- "COCO/DAND5 inhibits developmental and pathological ocular angiogenesis” Popovic and Hooker et al. EMBO Molecular Medicine, 2021.
- "Interleukin-1β induces pericyte apoptosis via the NF-κB pathway in diabetic retinopathy” Jang-Hyuk Yun Biochemical and Biophysical Research Communications, 2021.
- "PSF functions as a repressor of hypoxia-induced angiogenesis by promoting mitochondrial function” Dong et al. Cell Communication and Signaling, 2021.
- "Pathological angiogenesis in retinopathy engages cellular senescence and is amenable to therapeutic elimination via BCL-xL inhibition” Crespo-Garcia et al. Cell Metabolism, 2021.
- "A 60% edible ethanolic extract of Ulmus davidiana inhibits vascular endothelial growth factor-induced angiogenesis” Park, J., Kim, H.-O. and Park, K.-H et al. Molecules, 2021.
- "Hyperglycemia-induced VEGF and ROS production in retinal cells is inhibited by the mTOR inhibitor, rapamycin" Kida et al. Scientific Reports, 2021.
- "Excess adiponectin in eyes with progressive ocular vascular diseases” Nishinaka and Nakamura et al. FASEB Journal, 2021.
- "miR-4319 inhibited retinoblastoma cells proliferation, migration, invasion and EMT progress via suppressing CD147 mediated MMPs expression” Wu et al. Journal of Molecular Histology, 2021.
- "RNA-Seq analysis reveals gene expression changes induced by IL-6 trans-signaling activation in retinal endothelial cells” Robinson et al. Cytokine, 2021.
- "Hydrogen Sulfide: A Potential Therapeutic Target in the Development of Diabetic Retinopathy” Mohammad et al. IOVS, 2020.
- "Involvement of Müller Glial Autoinduction of TGF-β in Diabetic Fibrovascular Proliferation Via Glial–Mesenchymal Transition” Wu et al. IOVS, 2020.
- "Human plasminogen-derived N-acetyl-Arg-Leu-Tyr-Glu antagonizes VEGFR-2 to prevent blood-retinal barrier breakdown in diabetic mice” Park et al. Biomedicine & Pharmacotherapy, 2020.
- "Disruption of profilin1 function suppresses developmental and pathological retinal neovascularization” Lau et al. Journal of Biological Chemistry, 2020.
- "Stress Signal Regulation by Na/K-ATPase As a New Approach to Promote Physiological Revascularization in a Mouse Model of Ischemic Retinopathy” Wang et al. IOVS, 2020.
- "Effect of miR-515-5p on Proliferation and Drug Sensitivity of Retinoblastoma Cells” Yuan and Yan et al. Dove Medical Press, 2020.
- "Capilliposide B blocks VEGF-induced angiogenesis in vitro in primary human retinal microvascular endothelial cells” Han et al. Biomedicine & Pharmacotherapy, 2020.
- "N-Methyl-D-aspartate receptor activation, novel mechanism of homocysteine-induced blood–retinal barrier dysfunction” Tawfik et al. Journal of Molecular Medicine, 2020.
- "Loss of sphingosine 1-phosphate receptor 3 gene function impairs injury-induced stromal angiogenesis in mouse cornea” Yasuda et al. Laboratory Investigation, 2020.
- "Normal vitreous promotes angiogenesis via activation of Axl” Wu et al. FASEB Journal, 2020.
- "TNF-α signaling regulates RUNX1 function in endothelial cells” Whitmore et al. FASEB Journal, 2020.
- "Downregulation of CKS1B restrains the proliferation, migration, invasion and angiogenesis of retinoblastoma cells through the MEK/ERK signaling pathway" Zeng et al. International Journal of Molecular Medicine, 2019.
- "Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films" Calejo et al. Acta Biomater, 2019.
- "Myeloid SOCS3 Deficiency Regulates Angiogenesis via Enhanced Apoptotic Endothelial Cell Engulfment" Korovina et al. J Innate Immunity, 2019.
- "Endomucin restores depleted endothelial glycocalyx in the retinas of streptozotocin-induced diabetic rats" Niu et al. FASEB Journal, 2019.
- "Epigenetic Modifications Compromise Mitochondrial DNA Quality Control in the Development of Diabetic Retinopathy" Mohammad et al. IOVS, 2019.
- "Targeting pericyte–endothelial cell crosstalk by circular RNA-cPWWP2A inhibition aggravates diabetes-induced microvascular dysfunction" Chang Liu, Hui-Min Ge, Bai-Hui Liu, Rui Dong, et al. PNAS, 2019.
- "Annexin A2 upregulation protects human retinal endothelial cells from oxygen‑glucose deprivation injury by activating autophagy" Jiang & Xu, Experimental and Therapeutic Medicine, 2019.
- "MicroRNA-34a (miR-34a) mediates retinal endothelial cell premature senescence through mitochondrial dysfunction and loss of antioxidant activities" Thounaojam et al, Antioxidants, 2019.
- "Angiopoietin 1 attenuates interleukin-6-induced endothelial cell permeability through SHP-1" Yun et al, BBRC, 2019.
- "GRP78 translocation to the cell surface and O-GlcNAcylation of VE-Cadherin contribute to ER stress-mediated endothelial permeability" Lenin et al. Scientific Reports, 2019.
- "Functional regulation of an oxidative stress mediator, Rac1, in diabetic retinopathy" Mohammad et al. Molecular Neurobiology, 2019.
- "Development and characterization of an in vitro system of the human retina using cultured cell lines" Churm et al. Clinical & Experimental Ophthalmology, 2019.
- "RANKL blockade suppresses pathological angiogenesis and vascular leakage in ischemic retinopathy" Ock et al. BBRC, 2019.
- "Pro-angiogenic ginsenosides F1 and Rh1 inhibit vascular leakage by modulating NR4A1" Kang et al. Scientific Reports, 2019.
- "ASK1 induces retinal microvascular endothelial cell apoptosis through ER stress-associated pathway" Zou et al. Intl J Clinical Experimental Pathology, 2019.
- "Modulation of the p75 neurotrophin receptor using LM11A-31 prevents diabetes-induced retinal vascular permeability in mice via inhibition of inflammation and the RhoA kinase pathway" Elshaer et al. Diabetologia, 2019.
- "Serum miR‑338‑5p has potential for use as a tumor marker for retinoblastoma" Zhou and Li, Oncology Letters, 2019.
- "Enhancing retinal endothelial glycolysis by inhibiting UCP2 promotes physiologic retinal vascular development in a model of retinopathy of prematurity" Han et al. IOVS, 2019.
- "Improvement in diabetic retinopathy through protection against retinal apoptosis in spontaneously diabetic Torii rats mediated by ethanol extract of Osteomeles schwerinae C.K. Schneid" Kim et al. Nutrients, 2019.
- "Mitochondrial fusion and maintenance of mitochondrial homeostasis in diabetic retinopathy" Duraisamy et al. BBA Molecular Basis of Disease, 2019.
- "MicroRNA-145 regulates pathological retinal angiogenesis by suppresion of TMOD3" Liu et al. Nucleic Acids, 2019.
- "Pro-angiogenic ginsenosides F1 and Rh1 inhibit vascular leakage by modulating NR4A1" Kang et al. Scientific Reports, 2019.
- "Three-dimensional tubule formation assay as therapeutic screening model for ocular microvascular disorders" Shariatzadeh et al. Nature: Eye, 2018.
- "RF/6A Chorioretinal Cells Do Not Display Key Endothelial Phenotypes" Makin et al. IOVS, 2018.
- "Epac1 deacetylates HMGB1 through increased IGFBP-3 and SIRT1 levels in the retinal vasculature" Jiang et al. Molecular Vision, 2018.
- "Vascular protection of DPP-4 inhibitors in retinal endothelial cells in in vitro culture" Li et al. International Immunopharmacology, 2018.
- "3-Hydroxypyruvate destabilizes hypoxia inducible factor and induces angiostasis" Singh et al. IOVS, 2018.
- "Adaptor protein p66Shc: a link between cytosolic and mitochondrial dysfunction in the development of diabetic retinopathy" Mishra et al. Antioxidants & Redox Signaling, 2018.
- "Interaction of palmitate and LPS regulates cytokine expression and apoptosis through sphingolipids in human retinal microvascular endothelial cells" Lu et al. Experimental Eye Research, 2018.
- "Activation of the sweet taste receptor T1R3 by sucralose attenuates VEGF-induced vasculogenesis in a cell model of the retinal microvascular endothelium" Lizunkova et al. Graefe's Archive for Clinical and Experimental Ophthalmology, 2018.
- "Activation of the Notch‑Nox4‑reactive oxygen species signaling pathway induces cell death in high glucose‑treated human retinal endothelial cells" Jiao et al. Molecular Medicine Reports, 2018.
- "YAP via interacting with STAT3 regulates VEGF-induced angiogenesis in human retinal microvascular endothelial cells" Zhu and Liu et al, Experimental Cell Research, 2018.
- "PKA regulates HMGB1 through activation of IGFBP-3 and SIRT1 in human retinal endothelial cells cultured in high glucose" Liu et al. Inflammation Research, 2018.
- "Effects of LY294002 on the function of retinal endothelial cell in vitro" Di and Chen, Intl J Ophthalmology, 2018.
- "miR-215 controls proliferation, invasion, and apoptosis of human retinoblastoma cells by regulating RB1 expression" Zhang et al. Intl J Experimental Clinical Medicine, 2018.
- "Expression of miR‑204 in pediatric retinoblastoma and its effects on proliferation and apoptosis of cancer cells" Ding and Lu, Oncology Letters, 2018.
- "Epigenetics and regulation of oxidative stress in diabetic retinopathy" A.J. Duraisamy et al. IOVS, 2018.
- "Tigecycline as a dual inhibitor of retinoblastoma and angiogenesis via inducing mitochondrial dysfunctions and oxidative damage" Xiong et al. Scientific Reports, 2018.
- "SiRNA silencing of VEGF, IGFs, and their receptors in human retinal microvascular endothelial cells" Nicolau et al. American J Translational Research, 2018.
- "Fenofibrate exerts protective effects in diabetic retinopathy via inhibition of the ANGPTL3 pathway" Wang et al. IOVS, 2018.
- "Critical role of endoplasmic reticulum stress in chronic endothelial activation−induced visual deficits in tie2‐tumor necrosis factor mice" Lenin et al. J Cellular Biochemistry, 2018.
- "miR15a regulates NLRP3 inflammasome proteins in the retinal vasculature" Curtiss et al. Experimental Eye Research, 2018.
- "CD140b (PDGFRβ) Signaling in Adipose-Derived Stem Cells Mediates Angiogenic Behavior of Retinal Endothelial Cells" Periasamy et al. Regenerative Engineering and Translational Medicine, 2018.
- "MicroRNA-145 attenuates high glucose-induced oxidative stress and inflammation in retinal endothelial cells through regulating TLR4/NF-κB signaling" Hui and Yin, Life Sciences, 2018.
- "Cross-inhibition of Norrin and TGF-β signaling modulates development of retinal and choroidal vasculature" Seitz et al, Retinal Cell Biology, 2018.
- "The benzodiazepine anesthetic midazolam prevents hyperglycemia-induced microvascular leakage in the retinas of diabetic mice" Lee, et al. FASEB Journal, 2018.
- "Intravitreal implantable magnetic micropump for on-demand VEGFR-targeted drug delivery" Wang et al. J Controlled Release, 2018.
- "Vitamin D3 protects against diabetic retinopathy by inhibiting high-glucose-induced activation of the ROS/TXNIP/NLRP3 inflammasome pathway" Lu et al. Journal of Diabetes Research, 2018.
- "Role of endoplasmic reticulum stress in 12/15-lipoxygenase-induced retinal microvascular dysfunction in a mouse model of diabetic retinopathy" Elmasry et al. Diabetologia, 2018.
- "Artemesia annua extract prevents glyoxal-induced cell injury in retinal microvascular endothelial cells during glaucoma" Jiang et al. Tropical Journal of Pharmaceutical Research, 2018.
- "Metformin suppresses retinal angiogenesis and inflammation in vitro and in vivo" Han et al. PLoS One, 2018.
- "Gastrodin inhibits high glucose‑induced human retinal endothelial cell apoptosis by regulating the SIRT1/TLR4/NF‑κBp65 signaling pathway" Zhang et al. Molecular Medicine Reports, 2018.
- "Profilin-1 mediates microvascular endothelial dysfunction in diabetic retinopathy through HIF-1α-dependent pathway" Ding et al. Int J Clin Exp Pathology, 2018.
- "DNA methylation—a potential source of mitochondria DNA base mismatch in the development of diabetic retinopathy" Mishra and Kowluru, Molecular Neurobiology, 2018.
- "Characterization of site-specific phosphorylation of NF-κB p65 in retinal cells in response to high glucose and cytokine polarization" Shi and Berger, Mediators of Inflammation, 2018.
- "Human retinal endothelial cells and astrocytes cultured on 3-D scaffolds for ocular drug discovery and development" Beharry et al. Prostaglandins and Other Lipid Mediators, 2018.
- "ANGPTL-4 induces diabetic retinal inflammation by activating Profilin-1" Lu et al. Experimental Eye Research, 2018.
- "Cathepsin D plays a role in endothelial–pericyte interactions during alteration of the blood–retinal barrier in diabetic retinopathy" Monickaraj et al. FASEB Journal, 2018.
- "Beta-adrenergic receptor agonists attenuate pericyte loss in diabetic retinas through Akt activation" Yun et al. FASEB Journal, 2018.
- "miRNA15a regulates insulin signal transduction in the retinal vasculature" Jiang et al. Cellular Signalling, 2018.
- "miR-539-5p inhibits experimental choroidal neovascularization by targeting CXCR7" Feng et al. FASEB Journal, 2018.
- "MnTBAP or catalase is more protective against oxidative stress in human retinal endothelial cells exposed to intermittent hypoxia than their co-administration" Quan et al, Reactive Oxygen Species, 2017.
- "Calcium influx through TRPV4 channels modulates the adherens contacts between retinal microvascular endothelial cells" Phuong et al. J Physiology, 2017.
- "MicroRNA‐containing extracellular vesicles released from endothelial colony‐forming cells modulate angiogenesis during ischaemic retinopathy" Dellett et al. Journal of Cellular and Molecular Medicine, 2017.
- "Hyperhomocysteinemia alters retinal endothelial cells barrier function and angiogenic potential via activation of oxidative stress" Mohamed et al. Scientific Reports, 2017.
- "Zika virus infection of cellular components of the blood-retinal barriers: implications for viral associated congenital ocular disease" Roach and Alcendor, Journal of Neuroinflammation, 2017.
- "Activation of the TXNIP/NLRP3 inflammasome pathway contributes to inflammation in diabetic retinopathy: a novel inhibitory effect of minocycline" Chen et al. Inflammation Research, 2017.
- "Contacting co-culture of human retinal microvascular endothelial cells alters barrier function of human embryonic stem cell derived retinal pigment epithelial cells" Skottman et al. Experimental Cell Research, 2017
- "AAV-CRISPR/Cas9–mediated depletion of VEGFR2 blocks angiogenesis in vitro" Wu et al. IOVS, 2017.
- "Association of HMGB1 with oxidative stress markers and regulators in PDR" Abu El-Asrar and Alam et al. Molecular Vision, 2017.
- "STAT3-mediated activation of miR-21 is involved in down-regulation of TIMP3 and neovascularization in the ischemic retina" Gutsavea et al. Oncotarget, 2017.
- "Crosstalk between histone and DNA methylation in regulation of retinal matrix metalloproteinase-9 in diabetes" Duraisamy et al. IOVS, 2017.
- "Therapeutic effect of novel single-stranded RNAi agent targeting periostin in eyes with retinal neovascularization" Nakama et al. Molecular Therapy - Nucleic Acids, 2017.
- "Zika virus infects cells lining the blood-retinal barrier and causes chorioretinal atrophy in mouse eyes" Singh et al. JCI Insight, 2017.
- "Phenotype-based discovery of 2-[(E)-2-(Quinolin-2-yl)vinyl)phenol as a novel regulator of ocular angiogenesis" Reynolds et al, J Biological Chemistry, 2016.
- "A novel angiogenic peptide, ΔADT: A truncated adrenotensin peptide revealed by secretory peptidome analysis of human retinal pericytes" Okumura et al. BioScience Trends, 2016.
- "miR-146a attenuates inflammatory pathways mediated by TLR4/NF-κB and TNFα to protect primary human retinal microvascular endothelial cells grown in high glucose" Ye and Steinle, Mediators of Inflammation, 2016.
- "Src tyrosine kinase regulates the stem cell factor–induced breakdown of the blood–retinal barrier" Im et al. Molecular Vision, 2016.
- "Lymphoblastoid cell lines as a tool to study inter-individual differences in the response to glucose" Grassi et al. PLoS One, 2016.
- "Tenascin-C promotes angiogenesis in fibrovascular membranes in eyes with proliferative diabetic retinopathy" Kobayashi et al. Molecular Vision, 2016.
Standard Tests
TESTS | RESULTS |
HIV Serologic Test (donor level HIV AB EIA) | Negative |
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
TESTS | RESULTS |
Bacterial Sterility (culture method) by independent lab | Pass |
Fungal Sterility (culture method) by independent lab | Pass |
Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
TESTS | RESULTS |
Cytoplasmic VWF / Factor VIII | > 95% positive by immunofluorescence |
Cytoplasmic uptake of Di-I-Ac-LDL | > 95% positive by immunofluorescence |
Cytoplasmic CD31 | > 95% positive by immunofluorescence |
GFAP (Glial Fibrillary Acidic Protein) | < 1% by immunofluorescence |
Glutamine Synthetase | < 1% by immunofluorescence |
NG2 (Neural/Glial Antigen 2) | < 1% by immunofluorescence |
PDGFR-beta (Platelet Derived Growth Factor Receptor beta) | < 1% by immunofluorescence |
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Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.