Primary Human Arterial Endothelial Cells (Pooled) (CSC 2A0)
Primary Human Arterial Endothelial Cells (Pooled) (CSC 2A0) are isolated from umbilical arteries of multiple donors.
These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask.
In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).
Each vial of cells is shipped with vials of Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.
These cells are qualified for use with:
|HIV Serologic Test (donor level HIV AB EIA)||Negative|
|HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)||Negative|
|Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)||Negative|
|Cytoplasmic VWF / Factor VIII||> 95% positive by immunofluorescence|
|Cytoplasmic uptake of Di-I-Ac-LDL||> 95% positive by immunofluorescence|
- "A putative angiogenin receptor in angiogenin-responsive human endothelial cells" Hu et al. PNAS, 1997.