Primary Human Brain Pericytes (ACBRI 498) - Cell Systems
Primary Human Brain Pericyte Cells (ACBRI 498) were isolated by enzymatic dissociation of normal human cerebral cortex tissue. Isolated cells were not bound by antibodies during processing and remain antibody-free.
ACBRI 498 at Passage 3, plated on AttachmentFactorTM-coated flask, cultured in Cell Systems Complete Medium Kit with Serum and CultureBoost-RTM (4Z0-500-R).
ACBRI 498 at Passage 3 labeled with antibodies against pericyte markers desmin, PDGFR-b, NG2, and CD13.
These cells were originated using Cell Systems Complete Classic Medium (4Z0-500) with serum and CultureBoostTM. The culture was passaged using Complete Classic Medium with serum and CultureBoost-RTM (4Z0-500-R). They are available at Passage 3 [< 12 cumulative population doublings], cryopreserved in Cell Systems Cell Freezing MediumTM (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask. Each vial contains more than 1 x 106 cells. In addition to cryopreserved vials, these cells are also available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only). Each vial contains >1x106 cells.
Each vial of cells is shipped with Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.
These cells are qualified for use with:
|HIV Serologic Test||Negative|
|RPR Syphilis Test||Negative|
|Hepatitis B Serologic Test||Negative|
|Hepatitis C Serologic Test||Negative|
Cell Type Tests
Desmin pericyte marker
> 98% positive by immunofluorescence at P3
PDGFR-b pericyte marker
> 98% positive by immunofluorescence at P3 and P10
NG2 pericyte marker
|> 98% positive by immunofluorescence at P3 and P10|
|CD13 pericyte marker||
> 98% positive by immunofluorescence at P3 and P10
|a-SMA pericyte marker||> 98% positive by immunofluorescence at P10|
|CD31 endothelial cell marker||< 2% positive by immunofluorescence at P3 and P10|
|Uptake of DiI-Ac-LDL, endothelial cell test||< 2% positive by immunofluorescence at P3 and P10|
|MAP2 neuronal marker||< 2% positive by immunofluorescence at P3 and P10|
|Neurofilament neuronal marker||< 2% positive by immunofluorescence at P3 and P10|
|S100A4 fibroblast marker||< 2% positive by immunofluorescence at P3 and P10|
|GFAP astrocyte marker||< 2% positive by immunofluorescence at P3 and P10|
|GS astrocyte marker||< 2% positive by immunofluorescence at P3 and P10|
|CD11b microglial marker||< 2% positive by immunofluorescence at P3 and P10|
|IbaI microglial marker||< 2% positive by immunofluorescence at P3 and P10|
Some publications refer to ACBRI 499 Primary Human Brain Pericytes. This product has been replaced by ACBRI 498 Primary Human Brain Pericytes.
- "PDGFR-β restores blood-brain barrier functions in a mouse model of focal cerebral ischemia". Shen and Xu et al. J Cerebral Blood Flow & Metabolism, 2018.
- "Distinct contributions of astrocytes and pericytes to neuroinflammation Identified in a 3D human blood-brain barrier on a chip". Herland et al. PLoS One, 2016.
- "Atrasentan reduces albuminuria by restoring the glomerular endothelial glycocalyx barrier in diabetic nephropathy". Boels et al. Diabetes, 2016.
- "Retinal pericytes and cytomegalovirus infectivity: implications for HCMV-induced retinopathy and congenital ocular disease". Wilkerson et al. J Neuroinflammation, 2015.
- "Brain vascular pericytes following ischemia have multipotential stem cell activity to differentiate into neural and vascular lineage cells". Nakagomi et al. Stem Cells, 2015.
- "Inflammation-induced endothelial cell-derived extracellular vesicles modulate the cellular status of pericytes". Yamamoto et al. Scientific Reports, 2015.
- "Infection and upregulation of proinflammatory cytokines in human brain vascular pericytes by human cytomegalovirus". Alcendor et al. J Neuroinflammation, 2012.
- "PDE4 regulates tissue plasminogen activator expression of human brain microvascular endothelial cells". Yang et al. Thrombosis Research, 2012.
- "Expressions and roles of AMIGO gene family in vascular endothelial cells". Hossain et al. Intl J Bioscience Biochemistry Bioinformatics, 2012.
- "PDGF receptor β signaling in pericytes following ischemic brain injury". Arimura et al. Current Neurovascular Research, 2012.
- "Neurotrophin production in brain pericytes during hypoxia: A role of pericytes for neuroprotection". Ishitsuka et al. Microvascular Research, 2012.
- "Astrocytes and pericytes cooperatively maintain a capillary-like structure composed of endothelial cells on gel matrix". Itoh et al. Brain Research, 2011.
- "Astrocytes and pericytes differentially modulate blood-brain barrier characteristics during development and hypoxic insult". Al Ahmad et al. J Cerebral Blood Flow Metabolism, 2011.
- "Coordinated regulation of angiopoietin-1 and vascular endothelial growth factor by arsenite in human brain microvascular pericytes: Implications of arsenite-induced vascular dysfunction". Park et al. Toxicology, 2009.
- "Differential expression of stromal cell-derived factor 1 in human brain microvascular endothelial cells and pericytes involves histone modifications". Seo et al. BBRC, 2009.
- "Inhibition of transforming growth factor-β production in brain pericytes contributes to cyclosporin A-induced dysfunction of the blood-brain barrier". Takata et al. Cellular Molecular Neurobiology, 2007.
- "Brain endothelial homeostasis regulation by pericytes". Kim et al. J Cerebral Blood Flow Metabolism, 2006.
- "Glioma cells under hypoxic conditions block the brain microvascular endothelial cell death induced by serum starvation". Ueda et al. J Neurochemistry, 2005.
- "Novel splice variants of the receptor for advanced glycation end-products expressed in human vascular endothelial cells and pericytes, and their putative roles in diabetes-induced vascular injury". Yonekura et al. Biochemical Journal, 2003.
- "Hypoxia down-regulates endostatin production by human microvascular endothelial cells and pericytes". Wu et al. BBRC, 2001.
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