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Primary Human Brain Pericytes (ACBRI 498) - Cell Systems

Regular price $735.00

Primary Human Brain Pericyte Cells (ACBRI 498) were isolated by enzymatic dissociation of normal human cerebral cortex tissue. Isolated cells were not bound by antibodies during processing and remain antibody-free.

ACBRI 498 at Passage 3, plated on AttachmentFactorTM-coated flask, cultured in Cell Systems Complete Medium Kit with Serum and CultureBoost-RTM (4Z0-500-R).

ACBRI 498 at Passage 3 labeled with antibodies against pericyte markers desmin, PDGFR-b, NG2, and CD13.

Cell Initiation

These cells were originated using Cell Systems Complete Classic Medium (4Z0-500) with serum and CultureBoostTM. The culture was passaged using Complete Classic Medium with serum and CultureBoost-RTM (4Z0-500-R). They are available at Passage 3 [< 12 cumulative population doublings], cryopreserved in Cell Systems Cell Freezing MediumTM (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask. Each vial contains more than 1 x 106 cells. In addition to cryopreserved vials, these cells are also available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only). Each vial contains >1x106 cells. 

Each vial of cells is shipped with Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.

Companion Products

These cells are qualified for use with:

Complete Classic Medium Kit with Serum and CultureBoost™
Complete Classic Medium Kit with Serum and CultureBoost™

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Passage Reagent Group
Passage Reagent Group™

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The System | All-in-One Kit
The System with CultureBoost-R™ (CSS-A103)

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Standard Tests

    TEST RESULTS
    HIV Serologic Test 
    Negative
    RPR Syphilis Test Negative
    Hepatitis B Serologic Test Negative
    Hepatitis C Serologic Test Negative

    Cell Type Tests

    MARKER RESULTS
    Desmin pericyte marker
    > 98% positive by immunofluorescence at P3
    PDGFR-b pericyte marker
    > 98% positive by immunofluorescence at P3 and P10
    NG2 pericyte marker
    > 98% positive by immunofluorescence at P3 and P10
    CD13 pericyte marker
    > 98% positive by immunofluorescence at P3 and P10
    a-SMA pericyte marker > 98% positive by immunofluorescence at P10
    CD31 endothelial cell marker < 2% positive by immunofluorescence at P3 and P10
    Uptake of DiI-Ac-LDL, endothelial cell test < 2% positive by immunofluorescence at P3 and P10
    MAP2 neuronal marker < 2% positive by immunofluorescence at P3 and P10
    Neurofilament neuronal marker < 2% positive by immunofluorescence at P3 and P10
    S100A4 fibroblast marker < 2% positive by immunofluorescence at P3 and P10
    GFAP astrocyte marker < 2% positive by immunofluorescence at P3 and P10
    GS astrocyte marker < 2% positive by immunofluorescence at P3 and P10
    CD11b microglial marker < 2% positive by immunofluorescence at P3 and P10
    IbaI microglial marker < 2% positive by immunofluorescence at P3 and P10

     

     

    Citations

    Some publications refer to ACBRI 499 Primary Human Brain Pericytes. This product has been replaced by ACBRI 498 Primary Human Brain Pericytes.

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