Primary Human Cardiac Fibroblast Cells (ACBRI 5118)
Primary Human Cardiac Fibroblast Cells (ACBRI 5118) were initiated by centrifugal counterflow elutriation of dispase dissociated human cardiac muscle.
These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask.
In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).
Each vial of cells is shipped with vials of Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.
These cells are qualified for use with:
HIV Serologic Test (donor level HIV AB EIA)
|HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab)||Negative|
|Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)||Negative|
Cytoplasmic Vimentin Intermediate Filaments > 95% positive by immunofluorescence
> 95% positive by immunofluorescence
- "Role of SGLT1 in high glucose level-induced MMP-2 expression in human cardiac fibroblasts" Meng et al. Molecular Medicine Reports, 2018
- "Roles of transient receptor potential canonical (TRPC) channels and reverse-mode Na+/Ca2+ exchanger on cell proliferation in human cardiac fibroblasts: Effects of transforming growth factor β1" Ikeda et al. Elsevier, 2013
- "FOXO3A Regulates Peroxiredoxin III Expression in Human Cardiac Fibroblasts" Chiribau et al. Journal of Biological Chemistry, 2008.
- "NAD(P)H Oxidase 4 Mediates Transforming Growth Factor-beta 1- Induced Differentiation of Cardiac Fibroblasts Into Myofibroblasts" Cucoranu et al. Circulation Research, 2005.
Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.