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Primary Human Retinal Microvascular Endothelial Cells (ACBRI 181) - Cell Systems

Regular price $735.00

Cell Systems ACBRI 181 Primary Human Microvascular Endothelial Cells

Primary Human Retinal Microvascular Endothelial Cells (ACBRI 181) are initiated by elutriation of dispase dissociated normal human retinal tissue.

Cell Initiation 

These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm² flask. Each vial contains more than 1 x 10cells.

In addition to cryopreserved vial, these cells are also available in 25cm² and 75cm² proliferating cell culture flasks (US domestic market only).

Companion Products 

Each vial of cells is shipped to customer with Bac-Off® (antibiotic), and CultureBoost™ (animal derived growth factors) or CultureBoost-R (human recombinant growth factors)

These cells are qualified for use with:

Complete Classic Medium Kit with Serum and CultureBoost™
Complete Classic Medium Kit with Serum and CultureBoost™

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The System | All-in-One Kit
The System™

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Passage Reagent Group
Passage Reagent Group™

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Standard Tests

      TEST RESULTS
      HIV Serologic Test (donor level HIV AB EIA) Negative
      HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) Negative
      Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level)
      Negative
      Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) Negative

      Miscellaneous Tests

      TEST RESULTS
      Cytoplasmic VWF / Factor VIII  > 95% positive by immunofluorescence
      Cytoplasmic uptake of Di-I-Ac-LDL  > 95% positive by immunofluorescence
      Cytoplasmic CD31
      > 95% positive by immunofluorescence
      GFAP (Glial Fibrillary Acidic Protein)
      < 1% by immunofluorescence
      Glutamine Synthetase 
      < 1% by immunofluorescence
      NG2 (Neural/Glial Antigen 2)
      < 1% by immunofluorescence
      PDGFR-beta (Platelet Derived Growth Factor Receptor beta)
      < 1% by immunofluorescence

      Characterization in Peer-Reviewed Literature

      Cell Systems Primary Human Retinal Microvascular Endothelial Cells (HREC) express hallmark endothelial cell markers such as PECAM1/CD31 (A), von Willebrand Factor (B), and VE-Cadherin/CDH5 (C); and treatment with TNF-alpha induces expression of endothelial-cell specific protein E-selectin (D) as measured by mRNA abundance. In contrast, the widely-used immortalized cell line RF/6A does not share hallmark endothelial cell characteristics. Modified with permission from Figures 1-3, Makin et al. IOVS (2018) 59:5795-5802.

      Citations:


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