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Primary Human Aortic Endothelial Cells (ACBRI 375)

Regular price $735.00

Primary Human Aortic Endothelial Cells (ACBRI 375) were initiated from normal human descending aorta (aortic arch to renal artery). 

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Cell Initiation

These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask. Each vial contains more than 1 x 10cells. 

In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).

Companion Products

Each vial of cells is shipped with Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.

These cells are qualified for use with:

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Complete Classic Medium Kit with Serum and CultureBoost™
Complete Classic Medium Kit with Serum and CultureBoost™

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The System | All-in-One Kit
The System™

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Passage Reagent Group
Passage Reagent Group™

Standard Tests

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TEST
RESULTS
HIV Serologic Test (donor level HIV AB EIA)
Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)
Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)
Negative

Miscellaneous Tests

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TEST
RESULTS
Inducible expression of CD 62E (E-Selectin)
 > 90% positive by immunofluorescence
Cytoplasmic VWF / Factor VIII
> 95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL
> 95% positive by immunofluorescence

Citations

Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient  Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization. 

Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.

Please read our Terms and Conditions