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Primary Human Brain Microvascular Endothelial Cells (ACBRI 376)⎢Cell Systems

Regular price $735.00

Primary Human Brain Microvascular Endothelial Cells (ACBRI 376) were initiated by elutriation of dispase dissociated normal human brain cortex tissue.

ACBRI 376 3 days after plating, plated with Attachment Factor™, fed using Cell Systems Complete Medium Kit With Serum and CultureBoost-R™ (4Z0-500-R)

Digital Fluorescence Images (direct flourescence 400X, indirect immunofluoresence 6300X) @ Passage 5

 

Digital Immunofluorescence ZO-1 mAb (6300X) @ Passage 5

 

Response to recombinant human IL-1ß stimulation in a PMN adherence assay @ P5 in vitro.

Cell Initiation

These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).

Additional Information

These cells are involved in the blood-brain barrier, HIV-Aids pathology, inflammation, and interact in vivo with astrocyte cells (ACBRI 371) and pericyte cells (ACBRI 499)

Functional Test: IL-1ß Stimulated Leukocyte Adherence 

Primary Brain Microvascular Cells can provide a useful tool for adherence, transport and permeability studies of the blood-brain-barrier (BBB).  ACBRI 376 cells demonstrate particular markers of differentiation (interdigitated cell contact, desmosomes, (Z0-1 protein epitopes) similar to those observed in vivo.  The morphological and immunofluorescent markers detailed for ACBRI 376 are suggestive of a differentiated cell monolayer that can be used to study properties of the human BBB.  These cells are extensively used in studies of HIV transmission and AIDS-related BBB dynamics.

In a functional test, ACBRI 376 monolayers in Cell Systems Serum-Free Medium were tested for IL-1ß stimulated leukocyte adherence.  Human leukocyte adherence was specific and dose-dependent as demonstrated by pretreatment of the microvascular monolayer with soluble IL-1ß receptor.  Nonspecific leukocyte adherence caused by phorbol ester was the positive control.  Polymorphonuclear leukocytes (PMN) were freshly isolated from peripheral blood by elutriation.

Companion Products

Each vial of cells is shipped with Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.

These cells are qualified for use with:

Complete Classic Medium Kit with Serum and CultureBoost™
Complete Classic Medium Kit with Serum and CultureBoost™

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The System | All-in-One Kit
The System™

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Passage Reagent Group
Passage Reagent Group™

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Standard Tests

    TEST RESULTS
    HIV Serologic Test (donor level HIV AB EIA)
    Negative
    HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)
    Negative
    Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level)
    Negative
    Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) Negative

    Miscellaneous Tests

    TEST RESULTS
    Cytoplasmic VWF / Factor VIII
    > 95% positive by immunofluorescence
    Cytoplasmic uptake of Di-I-Ac-LDL
    > 95% positive by immunofluorescence

    Citations

        Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization. 

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