These antibody-free human primary cells were initiated by elutriation of dispase-dissociated normal human brain cortex tissue without the use of positive selection of antibodies. 

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Primary Human Glomerular Mesangial Cells (ACBRI 127)

  • PRODUCT INFO
  • CITATIONS
  • TESTS
  • RESEARCH
  • DOCUMENTATION

Increase Biological Relevance with Human Primary Cells

Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).

  • Isolated from normal, healthy donor tissue
  • Available at Passage 3 (<12 cumulative population doublings)
  • Each vial contains approximately 1 x 106 cells
  • Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
  • Available in reserved lots to enhance consistency in your research program

Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.


A Selection of Citations for ACBRI 376 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.

 


Standard Tests

TESTS RESULTS

HIV Serologic Test (donor level HIV AB EIA)

Negative            

HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab)

Negative

Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level)

Negative

Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)

Negative

Cell Markers and Functional Tests

MARKER RESULTS

CD31

> 98% positive by immunofluorescence

VWF / Factor VIII

> 95% positive by immunofluorescence

Uptake of Di-I-Ac-LDL

> 95% positive by immunofluorescence


Immunofluorescence Imagery and Characterization in Peer-Reviewed Literature

 

ACBRI 376 cells express endothelial cell markers including CD31 and von Willebrand Factor. They also take up DiI-Ac-LDL in an endothelial cell functional assay.

 

ACBRI 376 cells express ZO-1 indicating the formation of tight junctions.

 

 

Modified with permission from Storm et al EMBO Molecular Medicine (2019) 11:e9164.

Figure S1. Characterisation of HBMEC (Cell Systems ACBRI 376) at number of passages, as measured by flow cytometry. Unlabelled cells are depicted in grey. Blue = passage 5, light green = passage 7 (only in B), red = passage 8, dark green = passage 9. A, Uptake of DiI-labelled acetylated low density lipoprotein. B, Levels of CD31 expression. C, Upregulation of ICAM-1 by 10ng/ml TNF; dotted lines represent unstimulated labelled cells and the continuous lines represent the TNF-stimulated cells. D, Levels of EPCR expression on TNF-stimulated HBMEC. E, Levels of CD36 expression on TNF-stimulated HBMEC.

 

 

Reprinted with permission from Smits and Mir et al. PLoS ONE (2011) 6:e16282. Figure 5. Inhibition of EZH2 reduces endothelial tubule formation in vitro. (A) HBMVECs (ACBRI 376) were cultured on Matrigel coated plates in EBM, EGM, or EBM supplemented with U87-GFP cells. Scale bar  = 450 µm. Inhibition of EZH2 in HBMVECs, either by transfection with pre-miR-101, EZH2 siRNA, or treatment with DZNep significantly reduced tubule formation as compared to control cells. Tubule formation was assessed as tubule length and branching. (B) Quantitation of tubule length and branching using ImageJ software. (n = 3) Error bars indicate s.d. *p<0.05, ***p<0.001, t test.

 

 

Reprinted with permission from Smits and Mir et al. PLoS ONE (2011) 6:e16282. Figure 6. Inhibition of EZH2 reduces endothelial migration and invasion in vitro. (A) HBMVEC (ACBRI 376) monolayer cultures were scratched. Images were acquired directly after scratching (t = 0) and 24 h later (t = 24). The migration front is indicated by the dashed lines. Scale bar  = 450 µm. Inhibition of EZH2 in HBMVECs, either by transfection with pre-miR-101, EZH2 siRNA or treatment with DZNep significantly reduced migration as compared to control. (B) Quantitation of endothelial cell migration into the scratched area using ImageJ software. (C and D) HBMVECs were transfected with pre-miR-101, EZH2 siRNA, non-related control molecules, or treated with DZNep, incubated on a Transwell system and subsequently analyzed for invasion capability. EZH2 inhibition, either through pre-miR-101, EZH2 siRNA or DZNep, significantly decreased invasion as shown by Hoechst staining. Scale bar  = 225 µm. (n = 3) Error bars indicate s.d. *p<0.05, ***p<0.001, t test.

 

 

Reprinted with permission from Niego et al. PLoS ONE (2017) 12: e0177332.

Figure 5. Only KD025, but not fasudil, protects brain endothelial cells (ACBRI 376) from rt-PA and plasmin. (A) Small molecule KD025 concentration-dependently blocks permeability changes in primary human brain microvascular endothelial cells (ACBRI 376 BECs; cultured without astrocytes) treated for 6 h with DMSO as control or with rt-PA (25nM) and human plasminogen (plgn; 100nM), with or without KD025 (2 and 20μM). n = 4. (B) Comparison of selective ROCK-2 inhibition by KD025 (20μM) versus non-selective ROCK inhibition by fasudil (HA1077; 2, 20 and 100μM) against rt-PA+plasminogen (25nM+100nM, respectively) in BECs, as assessed 6 h post stimulation. HA1077 displays no protective capacity in BECs. n = 3. Bars represent mean±SEM. **P<0.01 compared to all other groups, ***P<0.001 compared to t-PA+Plgn by one-way ANOVA with Tukey’s post hoc analysis. ## P<0.01 compared to DMSO control and P = 0.06 by two-tailed paired t-test. (C) Representative phase-contrast micrographs (top panels) and double immunofluorescence images of zonula occludens 1 (ZO-1, representing tight junctions (TJs); white) and nuclei (Hoechst; red) (bottom panels) of ACBRI 376 BECs stimulated for 6 h with DMSO (control) or with rt-PA+plasminogen (25nM+100nM, respectively), in the presence or absence of KD025 (20μM). KD025 attenuates morphological changes (arrows) and preserves TJs in endothelial cells. Asterisks depict areas with degraded TJs. Scale bars = 200μm.


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