Primary Human Brain Microvascular Endothelial Cells (ACBRI 376)⎢Cell Systems
Primary Human Brain Microvascular Endothelial Cells (ACBRI 376) were initiated by elutriation of dispase dissociated normal human brain cortex tissue.
ACBRI 376 3 days after plating, plated with Attachment Factor™, fed using Cell Systems Complete Medium Kit With Serum and CultureBoost-R™ (4Z0-500-R)
These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask.
In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).
These cells are involved in the blood-brain barrier, HIV-Aids pathology, inflammation, and interact in vivo with astrocyte cells (ACBRI 371) and pericyte cells (ACBRI 499)
Functional Test: IL-1ß Stimulated Leukocyte Adherence
Primary Brain Microvascular Cells can provide a useful tool for adherence, transport and permeability studies of the blood-brain-barrier (BBB). ACBRI 376 cells demonstrate particular markers of differentiation (interdigitated cell contact, desmosomes, (Z0-1 protein epitopes) similar to those observed in vivo. The morphological and immunofluorescent markers detailed for ACBRI 376 are suggestive of a differentiated cell monolayer that can be used to study properties of the human BBB. These cells are extensively used in studies of HIV transmission and AIDS-related BBB dynamics.
In a functional test, ACBRI 376 monolayers in Cell Systems Serum-Free Medium were tested for IL-1ß stimulated leukocyte adherence. Human leukocyte adherence was specific and dose-dependent as demonstrated by pretreatment of the microvascular monolayer with soluble IL-1ß receptor. Nonspecific leukocyte adherence caused by phorbol ester was the positive control. Polymorphonuclear leukocytes (PMN) were freshly isolated from peripheral blood by elutriation.
Each vial of cells is shipped with Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.
These cells are qualified for use with:
HIV Serologic Test (donor level HIV AB EIA)
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)
Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level)
|Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)||Negative|
Cytoplasmic VWF / Factor VIII
> 95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL
> 95% positive by immunofluorescence
- "Tetrahydroxy stilbene glucoside ameliorates H2O2-induced human brain microvascular endothelial cell dysfunction in vitro by inhibiting oxidative stress and inflammatory responses"(Zhao Jiang, 2017)
- "Overexpression of pregnane X and glucocorticoid receptors and the regulation of cytochrome P450 in human epileptic brain endothelial cells" (Chaitali Ghosh,2017)
- "Immune activated monocyte exosomes alter microRNAs in brain endothelial cells and initiate an inflammatory response through the TLR4/MyD88 pathway" (Pranjali Dalvi, 2017)
- "Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke" (Kotaro Tatebayashi, 2017)
- "Long noncoding RNA MALAT1 inhibits apoptosis induced by oxygetn-glucose deprivation and reoxygenation in human brain microvascular endothelial cells" (Jia-Win Xin, 2017)
- "Scanning electron microscopy of the adhesion of Treponema pallidum subspecies pallidum (Nichol strain) to human brain microvascular endothelial cells in vitro" (F. Wu, 2017)
- "Selective inhibition of brain endothelial Rho-kinase-2 provides optimal protection of an in vitro blood-brain barrier from tissue-type plasminogen activator and plasmin" (Be’eri Niego, 2017)
- "Different Regulation of p53 Expression by Cadmium Exposure in Kidney, Liver, Intestine, Vasculature, and Brain Astrocytes" (Jin-Yong Lee, 2016)
- "Endothelial progenitor cells and neural progenitor cells synergistically protect cerebral endothelial cells from Hypoxia/reoxygenation-induced injury via activating the PI3K/Akt pathway"(Jinju Wang, 2016)
- "Distinct Contributions of Astrocytes and Pericytes to Neuroinflammation Identified in a 3D Human Blood-Brain Barrier on a Chip" (Anna Herland, 2016)
- "The Novel Methods for Analysis of Exosomes Released from Endothelial Cells and Endothelial Progenitor Cells" (Jinju Wang, 2016)
- "Rapid endothelial cytoskeletal reorganization enables early blood–brain barrier disruption and long-term ischaemic reperfusion brain injury" (Yejie Shi, 2016)
- "Soluble VCAM-1 impairs human brain endothelial barrier integrity via integrin α-4-transduced outside-in signaling" (Axel Haarmann, 2015)
- "Heregulin-HER3-HER2 signaling promotes matrix metalloproteinase-dependent blood-brain-barrier transendothelial migration of human breast cancer cell lines" (Majid Momeny, 2015)
- "Enhanced anticancer properties of lomustine in conjunction with docosahexaenoic acid in glioblastoma cell lines"(Harvey KA, 2015)
- "Recreating blood-brain barrier physiology and structure on chip: A novel neurovascular microfluidic bioreactor"(Jacuelyn A. Brown, 2015)
"JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals" (Dionna W. Williams, 2015)"Regulation of T-cell activation and migration by the kinase TBK1 during neuroinflammation"(Jiayi Yu, 2015)
Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.