These antibody-free human primary cells were initiated from normal human liver tissue by elutriation following dispase treatment of tissue without the use of positive selection of antibodies.
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- PRODUCT INFO
- CITATIONS
- TESTS
- DOCUMENTATION
Increase Biological Relevance with Human Primary Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Isolated from normal, healthy donor tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.
A Selection of Citations for ACBRI 566 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.
- "Evaluation of the utility of the Beta Human Liver Emulation System (BHLES) for CFSAN's regulatory toxicology program" Eckstru et al. Food and Chemical Toxicology, 2022.
- "LIF, a mitogen for choroidal endothelial cells, protects the choriocapillaris: implications for prevention of geographic atrophy" Li et al. EMBO Molecular Medicine, 2021.
- "Utilization of a model hepatotoxic compound, diglycolic acid, to evaluate Liver Organ-Chip performance and in vitro to in vivo concordance” Eckstrum et al. Food and Chemical Toxicology, 2020.
- "Three-Dimensional Human Liver-Chip Emulating Premetastatic Niche Formation by Breast Cancer-Derived Extracellular Vesicles” Kim et al. American Chemical Society, 2020.
- "Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts” Cohen et al. Scientific Reports, 2020.
- "Sustained perfusion of revascularized bioengineered livers heterotopically transplanted into immunosuppressed pigs” Shaheen et al. Nature Biomedical Engineering, 2019.
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"Primary human liver co-culture with flow and Kupffer cell integration on microfluidic liver-on-a-chip" Mazur (Dissertation thesis) 2015.
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"High levels of oncomiR-21 contribute to the senescence-induced growth arrest in normal human cells and its knock-down increases the replicative lifespan" Delago et al. Aging Cell, 2013.
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"Methods of enhancing lysosomal storage disease therapy by modulation of cell surface receptor density" Zhu and Chen, US Patent 7658916B2, 2010.
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"Cannabinoid‐2 receptor agonist HU‐308 protects against hepatic ischemia/reperfusion injury by attenuating oxidative stress, inflammatory response, and apoptosis" Rajesh et al. J Leukocyte Biology, 2007.
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"Cannabinoid-2 receptor mediates protection against hepatic ischemia/reperfusion injury" Batkai et al. FASEB Journal, 2007.
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"An adiponectin receptor, T-cadherin, was selectively expressed in intratumoral capillary endothelial cells in hepatocellular carcinoma: possible cross talk between T-cadherin and FGF-2 pathways" Adachi et al. Virchows Archiv, 2006.
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"Dexamethasone-mediated up-regulation of the mannose receptor improves the delivery of recombinant glucocerebrosidase to Gaucher macrophages" Zhu et al. J Pharmacology and Experimental Therapeutics, 2003.
Standard Tests
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
| TESTS | RESULTS |
| Bacterial Sterility (culture method) by independent lab | Pass |
| Fungal Sterility (culture method) by independent lab | Pass |
| Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
| TESTS | RESULTS |
| Inducible expression of CD 62E (E-Selectin) | > 90% positive by immunofluorescence |
| Cytoplasmic VWF / Factor VIII | > 95% positive by immunofluorescence |
| Cytoplasmic uptake of Di-I-Ac-LDL | > 95% positive by immunofluorescence |
COMPANION PRODUCTS:
OPTIMIZE YOUR RESEARCH
WITH HUMAN PRIMARY CELLS
Avoid the obstacles from immortalized cell lines or animal models and gain more pertinent insights into human cell biology.
Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.
Please read our Terms and Conditions