These antibody-free human primary cells were initiated by centrifugal elutriation following dispersal of normal retinal tissue with collagenase and dispase without the use of positive selection of antibodies.
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- PRODUCT INFO
- CITATIONS
- TESTS
- DOCUMENTATION
Increase Biological Relevance with Human Primary Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Isolated from normal, healthy donor tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.
A Selection of Citations for ACBRI 183 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.
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"METTL3-mediated N6-methyladenosine modification governs pericyte dysfunction during diabetes-induced retinal vascular complication" Suo, Liu, Zhang, Yao et al. Theranostics, 2022.
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"Human plasminogen-derived N-acetyl-Arg-Leu-Tyr-Glu antagonizes VEGFR-2 to prevent blood-retinal barrier breakdown in diabetic mice" Park et al. Biomedicine & Pharmacotherapy, 2021.
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"High Glucose Induces the Loss of Retinal Pericytes Partly via NLRP3-Caspase-1-GSDMD-Mediated Pyroptosis" Gan et al. BioMed Research International, 2020.
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"Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing" Saraswati et al. Nature Communications, 2019.
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"RANKL blockade suppresses pathological angiogenesis and vascular leakage in ischemic retinopathy" Ock et al. BBRC, 2019.
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"Targeting pericyte–endothelial cell crosstalk by circular RNA-cPWWP2A inhibition aggravates diabetes-induced microvascular dysfunction" Chang Liu, Hui-Min Ge, Bai-Hui Liu, Rui Dong, et al. PNAS, 2019.
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"Cathepsin D plays a role in endothelial–pericyte interactions during alteration of the blood–retinal barrier in diabetic retinopathy" Monickaraj et al. FASEB Journal, 2018.
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"Zika virus infection of cellular components of the blood-retinal barriers: implications for viral associated congenital ocular disease" Roach and Alcendor, Journal of Neuroinflammation, 2017.
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"Ursodeoxycholic acid attenuates endoplasmic reticulum stress-related retinal pericyte loss in streptozotocin-induced diabetic mice" Chung et al. J Diabetes Research, 2017.
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"Homonoia riparia and its major component, myricitrin, inhibit high glucose-induced apoptosis of human retinal pericytes" Pyun et al. Integrative Medicine Research, 2017.
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"A novel angiogenic peptide, ΔADT: A truncated adrenotensin peptide revealed by secretory peptidome analysis of human retinal pericytes" Okumura et al. BioScience Trends, 2016.
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"Pericyte plasticity-comparative investigation of the angiogenic and multilineage potential of pericytes from different human tissues" Herrmann et al. European Cells & Materials, 2016.
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"High glucose-induced retinal pericyte apoptosis depends on association of GAPDH and Siah1" Suarez et al. Journal of Biological Chemistry, 2015.
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"Retinal pericytes and cytomegalovirus infectivity: implications for HCMV-induced retinopathy and congenital ocular disease" Wilkerson et al. Journal of Neuroinflammation, 2015.
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"Gemigliptin, a dipeptidyl peptidase-4 inhibitor, inhibits retinal pericyte injury in db/db mice and retinal neovascularization in mice with ischemic retinopathy" Jung et al. BBA Molecular Basis of Disease, 2015.
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"Propranolol targets contractility of infantile hemangioma-derived pericytes" Lee et al. Br J Dermatol, 2014.
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"Ascorbic acid prevents high glucose-induced apoptosis in human brain pericytes" May et al. Biochem Biophys Res Commun, 2014.
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"Pericyte regulation of vascular remodeling through the CXC receptor 3" Bodnar et al. Arterioscler Thromb Vasc Biol, 2014.
Standard Tests
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
| TESTS | RESULTS |
| Bacterial Sterility (culture method) by independent lab | Pass |
| Fungal Sterility (culture method) by independent lab | Pass |
| Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
| TESTS | RESULTS |
| Cytoplasmic alpha-Actinin Filaments | > 80% positive by immunofluorescence |
| Cytoplasmic Desmin Intermediate Filaments | > 80% positive by immunofluorescence |
| Cytoplasmic VWF / Factor VIII | < 02% by immunofluorescence |
| Cytoplasmic uptake of Di-I-Ac-LDL | < 02% by immunofluorescence |
| NG2 (Neural/Glial Antigen 2) | >90% positive by immunofluorescence |
| PDGFR-beta (Platelet Derived Growth Factor Receptor beta) | >90% positive by immunofluorescence |
| GFAP (Glial Fibrillary Acidic Protein) | < 1% by immunofluorescence |
| Glutamine Synthetase | < 1% by immunofluorescence |
COMPANION PRODUCTS:
OPTIMIZE YOUR RESEARCH
WITH HUMAN PRIMARY CELLS
Avoid the obstacles from immortalized cell lines or animal models and gain more pertinent insights into human cell biology.
Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.
Please read our Terms and Conditions