These antibody-free human primary cells were initiated from normal human coronary artery without the use of positive selection of antibodies.
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- PRODUCT INFO
- CITATIONS
- TESTS
- DOCUMENTATION
Increase Biological Relevance with Human Primary Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Isolated from normal, healthy donor tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.
A Selection of Citations for ACBRI 377 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.
- "Differential procoagulatory response of microvascular, arterial and venous endothelial cells upon inflammation in vitro" Brandtner et al. Thrombosis Research, 2021.
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"RAGE and CCR7 mediate the transmigration of Zika-infected monocytes through the blood-brain barrier" Costa de Carvalho et al. Immunobiology, 2019.
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"Expression Profiling of the Ephrin (EFN) and Eph Receptor (EPH) Family of Genes in Atherosclerosis-Related Human Cells" Saamoto et al. J International Medical Research, 2011.
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"Endothelial microparticles induce formation of plateletaggregates via a von Willebrand factor/ristocetin dependentpathway, rendering them resistant to dissociation" Jimenez et al. Journal of Thrombosis and Haemostasis, 2005.
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"Role of ceramide in activation of stress-associated MAP kinases by minimally modified LDL in vascular smooth muscle cells" Elsevier, 2004.
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"Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis" Jimenez et al. Thrombosis Research, 2003.
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"Enhancement of sphingosine 1-phosphate-induced migration of vascular endothelial cells and smooth muscle cells by an EDG-5 antagonist" Osada et al. Elsevier, 2002.
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"P-285: Insulin enhances bradykinin -stimulated nitric oxide production in vascular endothelial cells" Matsumoto et al. American Journal of Hypertension, 2002.
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"P-202: Eicosapentaenoic acid (EPA) prevents vascular endothelial cell death induced by high glucose" Yamamoto et al. American Journal of Hypertension, 2002.
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"Identification of macrophage migration inhibitory factor as a potent endothelial cell growth-promoting agent released by ectopic human endometrial cells" Yang et al. The Journal of Clinical Endocrinology & Metabolism, 2000.
Standard Tests
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
| TESTS | RESULTS |
| Bacterial Sterility (culture method) by independent lab | Pass |
| Fungal Sterility (culture method) by independent lab | Pass |
| Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
| TESTS | RESULT |
| Inducible expression of CD 62E (E-Selectin) | > 90% positive by immunofluorescence |
| Cytoplasmic VWF / Factor VIII | > 95% positive by immunofluorescence |
| Cytoplasmic uptake of Di-I-Ac-LDL | > 95% positive by immunofluorescence |
COMPANION PRODUCTS:
OPTIMIZE YOUR RESEARCH
WITH HUMAN PRIMARY CELLS
Avoid the obstacles from immortalized cell lines or animal models and gain more pertinent insights into human cell biology.
Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.
Please read our Terms and Conditions