These antibody-free human primary cells were isolated from foreskins of multiple neonatal donors without the use of positive selection of antibodies.
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- PRODUCT INFO
- CITATIONS
- TESTS
- RESEARCH
- DOCUMENTATION
Increase Biological Relevance with Human Primary Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Isolated from normal, healthy donor tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.
A Selection of Citations for CSC 2F0 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.
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"Selective Inhibition of β-Catenin/Co-Activator Cyclic AMP Response Element-Binding Protein-Dependent Signaling Prevents the Emergence of Hapten-Induced Atopic Dermatitis-Like Dermatitis" Matsuda-Hirose et al. Annals of Dermatology, 2019
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"Influence of Fe3O4 Nanoparticles in Hydroxyapatite Scaffolds on Proliferation of Primary Human Fibroblast Cells" Maleki-Ghaleh et al.Journal of Materials Engineering and Performance, 2016.
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"Protective effects of pressurized xenon gas against cold damage in a cell monolayer" Seino et al.Kitasato Medical Journal, 2016.
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"Logical design of an anti-cancer agent targeting the plant homeodomain in Pygopus2" Ali et al. Cancer Science, 2016.
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"Zerumbone induces mitochondria-mediated apoptosis via increased calcium, generation of reactive oxygen species and upregulation of soluble histone H2AX in K562 chronic myelogenous leukemia cells" Rajan et al. Tumor Biology, 2015.
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"Corrosion and biological behavior of nanostructured 316L stainless steel processed by severe plastic deformation" Hajizadeh et al. Surface and Interface Analysis, 2015.
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"Pre-incubation with hyaluronan reduces cellular damage after cryopreservation in densely cultured cell monolayers" Iwama et al. Bio-Medical Materials and Engineering, 2014.
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"Protection of free radical-induced cytotoxicity by 2-O-α-d-glucopyranosyl-l-ascorbic acid in human dermal fibroblasts" Hanada et al.Bioscience, Biotechnology, and Biochemistry, 2013.
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"Cryoprotective Effect of Low-Molecular-Weight Hyaluronan on Human Dermal Fibroblast Monolayers" Ujihira et al. Cryoletters, 2010.
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"Investigation of the Influence of Cell Density of Human Fibroblasts Cryopreserved Inside Collagen Sponges at Various Cooling Rates" Matsumura et al. Cryoletters, 2007.
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"Zerumbone induced apoptosis in liver cancer cells via modulation of Bax/Bcl-2 ratio" Sharifah Sakinah et al. Cancer Cell International, 2007.
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"In vitro Mineralization by Mesenchymal Stem Cells Cultured on Titanium Scaffolds" Maeda et al. Journal of Biochemistry, 2007.
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"Assessment of Viability and Osteogenic Ability of Human Mesenchymal Stem Cells After Being Stored in Suspension for Clinical Transplantation" Muraki et al. Tissue Engineering, 2006.
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"In-Situ Visualization and Quantification of Mineralization of Cultured Osteogenetic Cells" Uchimura et al. Calcified Tissue International, 2003.
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"A rapid biosensor chip assay for measuring of telomerase activity using surface plasmon resonance" Maesawa et al. Nucleic Acids Research, 2003.
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"Novel anti-inflammatory actions of nobiletin, a citrus polymethoxy flavonoid on human synovial fibroblasts and mouse macrophages" Lin et al. Biochemical Pharmacology, 2003.
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"Zerumbone, a Southeast Asian ginger sesquiterpene, markedly suppresses free radical generation, proinflammartory protein production, and cancer cell proliferation accompanied by apaptosis:the alpha, beta unsaturated carbonyl group is a prerequisite" Murakami et al. Carcinogenesis, 2002.
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"Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhena asphodeloides Bunge" Takeda et al. Journal of Gastroenterology, 2001.
Standard Tests
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
| TESTS | RESULTS |
| Bacterial Sterility (culture method) by independent lab | Pass |
| Fungal Sterility (culture method) by independent lab | Pass |
| Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
| TESTS | RESULTS |
| Vimentin Intermediate Filaments | > 95% positive by immunofluorescence |
Cells Immunofluorescence Imagery
CSC 2F0 cells express fibroblast markers including Fibroblast Surface Protein (FSP) and S100A4.
COMPANION PRODUCTS:
OPTIMIZE YOUR RESEARCH
WITH HUMAN PRIMARY CELLS
Avoid the obstacles from immortalized cell lines or animal models and gain more pertinent insights into human cell biology.
Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.
Please read our Terms and Conditions