These antibody-free human primary cells were initiated by decapsulated glomeruli isolated from normal human kidney cortical tissue without the use of positive selection of antibodies.

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Primary Human Glomerular Microvascular Endothelial Cells Day 2 ACBRI 128

  • PRODUCT INFO
  • CITATIONS
  • TESTS
  • RESEARCH
  • DCUMENTATION

Increase Biological Relevance with Human Primary Cells

Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).

  • Isolated from normal, healthy donor tissue
  • Available at Passage 3 (<12 cumulative population doublings)
  • Each vial contains approximately 1 x 106 cells
  • Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
  • Available in reserved lots to enhance consistency in your research program

Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.

 


A Selection of Citations for ACBRI 128 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells

 


Standard Tests

TESTS RESULTS

HIV Serologic Test (donor level HIV AB EIA)

Negative            

HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab)

Negative

Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level)

Negative

Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)

Negative

Miscellaneous Tests

TESTS RESULTS

Inducible expression of CD 62E (E-Selectin)

> 90% positive by immunofluorescence

Cell adhesion protein CD31

> 95% positive by immunofluorescence

Cytoplasmic VWF / Factor VIII

> 95% positive by immunofluorescence

Cytoplasmic uptake of Di-I-Ac-LDL

> 95% positive by immunofluorescence


Immunofluorescence imagery and characterization in peer reviewed literature

 

ACBRI 128 at Passage 6, demonstrating uptake of fluorescently labeled DiI-Ac-LDL as functional test for endothelial cell identity.

 

 

 

Immunofluorescence staining showing expression of endothelial (EnC)-specific markers by human glomerular EnC (GEnC) cultured on coverslips. Magnification, x200.

 

 

Chart showing the effect of TNF-α on e-selectin expression by GEnC in a cell-based fluorescence immunoassay. GEnC were incubated with TNF-α at various concentrations or control medium for 6 h before fixing and labeling for e-selectin. E-selectin expression is proportional to fluorescence emission. Bars show mean ± SEM; n = 12; P < 0.001 by ANOVA.

 

 

Chart showing the results of separate experiments examining the effect of cAMP medium (containing 2 μM RO-20-1724 and 30 μM pCPT-cAMP) or thrombin (1 U/ml) on transendothelial electrical resistance (TEER) of GEnC monolayers after 1 h. Bars show mean ± SEM; n = 5, P = 0.006 for effect of cAMP; n = 8, P < 0.0005 for effect of thrombin, P values by t test. cAMP medium increased TEER by 34.4 Ω/cm2 compared with control, whereas thrombin decreased TEER by 14.8 Ω/cm2.

 

 

Graphs showing the effect of “cAMP medium” (containing 2 μM RO-20-1724 and 300 μM pCPT-cAMP; A) or thrombin (1 U/ml; B) on passage of FITC-BSA through GEnC monolayers over time. Points show mean ± SEM, n = 10, P = 0.003 for effect of cAMP; n = 10, P < 0.0005 for effect of thrombin by repeated measures [rm] ANOVA.

From Satchell et al (2004) J Am Soc Nephrology 15(3):566-574.


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Online Special: Save $200 with the Purchase of Primary Cells. Automatic discount at checkout.

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OPTIMIZE YOUR RESEARCH
WITH HUMAN PRIMARY CELLS

Avoid the obstacles from immortalized cell lines or animal models and gain more pertinent insights into human cell biology.

Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.

Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.

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