Primary Human Glomerular Microvascular Endothelial Cells (ACBRI 128) - Cell Systems
Primary Human Glomerular Microvascular Endothelial Cells (ACBRI 128) were initiated by decapsulated glomeruli isolated from normal human kidney cortical tissue.
ACBRI 128 at Passage 4, 3 days after plating, plated with Attachment Factor™, fed using Cell Systems Complete Medium Kit With Serum and CultureBoost-R™ (4Z0-500-R).
ACBRI 128 at Passage 6, demonstrating uptake of fluorescently labeled DiI-Ac-LDL as functional test for endothelial cell identity.
These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask.
In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).
Each vial of cells is shipped with vials of Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (recombinant human growth factors) at no additional cost.
HIV Serologic Test (donor level HIV AB EIA)
|HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab)||Negative|
|Hepatitis B (HBV) PCR Test (frozen cells)||Negative|
|Hepatitis C (HCV) PCR Test (frozen cells)||Negative|
|Mycoplasma spp. (frozen cells)||Negative|
|Inducible expression of CD 62E (E-Selectin)||> 90% positive by immunofluorescence|
Cell adhesion protein CD31
|> 95% positive by immunofluorescence|
Cytoplasmic VWF / Factor VIII
|> 95% positive by immunofluorescence|
Cytoplasmic uptake of Di-I-Ac-LDL
> 95% positive by immunofluorescence
Characterization in Peer-Reviewed Literature
Immunofluorescence staining showing expression of endothelial (EnC)-specific markers by human glomerular EnC (GEnC) cultured on coverslips. Magnification, x200.
Chart showing the effect of TNF-α on e-selectin expression by GEnC in a cell-based fluorescence immunoassay. GEnC were incubated with TNF-α at various concentrations or control medium for 6 h before fixing and labeling for e-selectin. E-selectin expression is proportional to fluorescence emission. Bars show mean ± SEM; n = 12; P < 0.001 by ANOVA.
Chart showing the results of separate experiments examining the effect of cAMP medium (containing 2 μM RO-20-1724 and 30 μM pCPT-cAMP) or thrombin (1 U/ml) on transendothelial electrical resistance (TEER) of GEnC monolayers after 1 h. Bars show mean ± SEM; n = 5, P = 0.006 for effect of cAMP; n = 8, P < 0.0005 for effect of thrombin, P values by t test. cAMP medium increased TEER by 34.4 Ω/cm2 compared with control, whereas thrombin decreased TEER by 14.8 Ω/cm2.
Graphs showing the effect of “cAMP medium” (containing 2 μM RO-20-1724 and 300 μM pCPT-cAMP; A) or thrombin (1 U/ml; B) on passage of FITC-BSA through GEnC monolayers over time. Points show mean ± SEM, n = 10, P = 0.003 for effect of cAMP; n = 10, P < 0.0005 for effect of thrombin by repeated measures [rm] ANOVA.
From Satchell et al (2004) J Am Soc Nephrology 15(3):566-574.
- "Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip" Musah et al. Nature Protocols, 2018
- "Brain microvascular endothelial cells exhibit lower activation of the alternative complement pathway than glomerular microvascular endothelial cells" Sartain et al. J Biological Chemistry, 2018
- "Tacrolimus Prevents von Willebrand Factor Secretion by Allostimulated Human Glomerular Endothelium" Beland et al. Am J Transplantation, 2018
- "Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip" Musah et al. Nature and Biological Engineering, 2017
- "The formation of quiescent glomerular endothelial cell monolayer in vitro is strongly dependent on the choice of extracellular matrix coating" Pajecka et al. Experimental Cell Research, 2017.
- "The human kidney capsule contains a functionally distinct mesenchymal stromal cell population" Leuning et al. PLoS One, 2017.
- "Nitrite Activates 5′AMP-Activated Protein Kinase-Endothelial Nitric Oxide Synthase Pathway in Human Glomerular Endothelial Cells" Miyamoto et al. Biol. & Pharma Bulletin, 2017.
- "Circulating Fibroblast Growth Factor-2, HIV-Tat, and Vascular Endothelial Cell Growth Factor-A in HIV-Infected Children with Renal Disease Activate Rho-A and Src in Cultured Renal Endothelial Cells" Das et al. PLoS One, 2016.
- "TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human GLomerular Endothelial Cells" Sartain et al. J Immunology, 2016.
- "TNF causes changes in glomerular endothelial permeability and morphology through a Rho and myosin light chain kinase-dependent mechanism" Xu et al. Physiological Reports, 2015.
- "Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings" Turner and Moake, PLoS One, 2015.
- "Adenosine Kinase Inhibition Protects The Kidney Against Streptozotocin-Induced Diabetes Through Anti-inflammatory and Anti-oxidant Mechanisms" Pye et al. Pharmacological Research, 2014.
- "Adenoviral E4 Gene Stimulates Secretion of Pigmental Epithelium Derived Factor (PEDF) that Maintains Long-term Survival of Human Glomerulus-derived Endothelial Cells" Jerebtsova et al. Molecular & Cellular Proteomics, 2012.
- "Vegfa Protects the Glomerular Microvasculature in Diabetes" Sivaskandarajah et al. Diabetes, 2012.
- "Suppression of Adiponectin by Aberrantly Glycosylated IgA1 in Glomerular Mesangial Cells In Vitro and In Vivo" Inoue et al. PLoS One, 2012.
- "Glucagon-like peptide-1 receptor agonist ameliorates renal injury through its anti-inflammatory action without lowering blood glucose level in a rat model of type 1 diabetes" Kodera et al. Diabetologia, 2011
- "Localization of dichlorofluorescin in cardiac myocytes: implications for assessment of oxidative stress" Swift and Sarvazyan, Am J Physiol Heart Circ Physiol; 2011.
- "Protective effect of photodegradation product of nifedipine against tumor necrosis factor alpha-induced oxidative stress in human glomerular endothelial cells" Fukuhara et al. J Med Invest, 2011.
- "The Alternatively Spliced Anti-Angiogenic Family of VEGF Isoforms VEGF xxxbin Human Kidney Development" Bevan et al. Nephron Physiology, 2008.
- "Glomerular angiotensinogen protein is enhanced in pediatric IgA nephropathy" Takamatsu et al. Pediatric Nephrology, 2008.
- "Conditionally immortalized human glomerular endothelial cells expressing fenestrations in response to VEGF" Satchell et al. Kidney International, 2006
- "Role of fibroblast growth factor-binding protein in the pathogenesis of HIV-associated helolytic uremic syndrome" Ray et al. Am JPhysiology, 2006.
- "Hemolytic uremic syndrome-associated Siga toxins promote endothelial-cell secretion and impair ADAMTS13 cleavage of unusually large von Willebrand factor multimers" Nolasco et al. Blood, 2005.
- "Endothelial microparticles induce formation of plateletaggregates via a von Willebrand factor/ristocetin dependentpathway, rendering them resistant to dissociation" Jimenez et al. Journal of Thrombosis and Haemostasis, 2005
- "Effect on high glucose on nitric oxide production and endothelial nitrict oxide synthase protein expression in human glomerular endothelial cells" Hoshiyama et al. Nephron Experimental Nephrology, 2003
- "Angopoietin 1 and Vascular Endothelial Growth Factor Modulate Human Glomerular endothelial Cell Barrier Properties" Satchell et al J Am Soc Nephrology, 2003.
- "Elevated endothelial microparticles in thrombotic thrombocytopenic purpura: findings from brain and renal microvascular cell culture and patients with active disease" Jiminez et al. British Journal of Haematology, 2001
- "Role of caspases 1 and 3 and Bcl‐2‐related molecules in endothelial cell apoptosis associated with thrombotic microangiopathies" Mitra et al. Am J Hematology, 1998
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