Primary Human Glomerular Microvascular Endothelial Cells (ACBRI 128)⎢Cell Systems
Primary Human Glomerular Microvascular Endothelial Cells (ACBRI 128) were initiated by decapsulated glomeruli isolated from normal human kidney cortical tissue.
"ACBRI 128 2 days after plating, plated with Attachment Factor™, fed using CS Complete Medium Kit With Serum and Cultureboost-R™ (4Z0-500-R)"
These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask.
In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).
Each vial of cells is shipped with Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.
HIV Serologic Test (donor level HIV AB EIA)
|HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)||Negative|
|Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)||Negative|
|Inducible expression of CD 62E (E-Selectin)||> 90% positive by immunofluorescence|
Cell adhesion protein CD 31
|> 95% positive by immunofluorescence|
Cytoplasmic VWF / Factor VIII
|> 95% positive by immunofluorescence|
Cytoplasmic uptake of Di-I-Ac-LDL
> 95% positive by immunofluorescence
- "The formation of quiescent glomerular endothelial cell monolayer in vitro is strongly dependent on the choice of extracellular matrix coating" (Kamilla Pajecka, 2017)
- "Circulating Fibroblast Growth Factor-2, HIV-Tat, and Vascular Endothelial Cell Growth Factor-A in HIV-Infected Children with Renal Disease Activate Rho-A and Src in Cultured Renal Endothelial Cells" (Jharna Das, 2016)
- "TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human GLomerular Endothelial Cells" (SE Sartain, 2016)
- "TNF causes changes in glomerular endothelial permeability and morphology through a Rho and myosin light chain kinase-dependent mechanism" (Chang Xu, Xiaoyan, 2015)
- "Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings" (Nancy A. Turner, 2015)
- "Adenosine Kinase Inhibition Protects The Kidney Against Streptozotocin-Induced Diabetes Through Anti-inflammatory and Anti-oxidant Mechanisms" (Chelsey Pye, 2014)
- "Adenoviral E4 Gene Stimulates Secretion of Pigmental Epithelium Derived Factor (PEDF) that Maintains Long-term Survival of Human Glomerulus-derived Endothelial Cells" (Marina Jerebtsova, 2012)
- "Vegfa Protects the Glomerular Microvasculature in Diabetes" (Gavasker A. Sivaskandarajah, 2012)
- "Suppression of Adiponectin by Aberrantly Glycosylated IgA1 in Glomerular Mesangial Cells In Vitro and In Vivo" (Tatsuyuki Inoue, 2012)
- "Localization of dichlorofluorescin in cardiac myocytes: implications for assessment of oxidative stress" (Luther M. Smith, 2011)
- "Protective effect of photodegradation product of nifedipine against tumor necrosis factor alpha-induced oxidative stress in human glomerular endothelial cells" (Edgar A. Jaimes, 2009)
- "The Alternatively Spliced Anti-Angiogenic Family of VEGF Isoforms VEGF xxxbin Human Kidney Development" (Heather S. Bevan, 2009)
- "Glomerular angiotensinogen protein is enhanced in pediatric IgA nephropathy" (Masanori Takamatsu, 2008)
- "Role of fibroblast growth factor-binding protein in the pathogenesis of HIV-associated helolytic uremic syndrome" (Patricio E. Ray, 2005)
- "Hemolytic uremic syndrome-associated Siga toxins promote endothelial-cell secretion and impair ADAMTS13 cleavage of unusually large von Willebrand factor multimers" (Leticia H. Nolasco, 2005)
- "Angopoietin 1 and Vascular Endothelial Growth Factor Modulate Human Glomerular endothelial Cell Barrier Properties" (Simon C. Satchell, 2004)
- "Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis" (Joaquin J. Jimenez, 2003)
Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.