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Primary Human Glomerular Microvascular Endothelial Cells (ACBRI 128)

Regular price $735.00

Primary Human Glomerular Microvascular Endothelial Cells (ACBRI 128) were initiated by decapsulated glomeruli isolated from normal human kidney cortical tissue.


ACBRI 128 at Passage 4, 3 days after plating, plated with Attachment Factor™, fed using Cell Systems Complete Medium Kit With Serum and CultureBoost-R™ (4Z0-500-R).

ACBRI 128 at Passage 6, demonstrating uptake of fluorescently labeled DiI-Ac-LDL as functional test for endothelial cell identity.

Cell Initiation

These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask. Each vial contains approximately 0.5 x 10E6 cells. 

In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).

Companion Products

Each vial of cells is shipped with vials of Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (recombinant human growth factors) at no additional cost.

Complete Classic Medium Kit with Serum and CultureBoost™
Complete Classic Medium Kit with Serum and CultureBoost™


The System | All-in-One Kit
The System™


Passage Reagent Group
Passage Reagent Group™


Standard Tests

    HIV Serologic Test (donor level HIV AB EIA)
    HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) Negative
    Hepatitis B (HBV) PCR Test (frozen cells) Negative
    Hepatitis C (HCV) PCR Test (frozen cells) Negative
    Mycoplasma spp. (frozen cells) Negative

    Miscellaneous Tests

    Inducible expression of CD 62E (E-Selectin)  > 90% positive by immunofluorescence
    Cell adhesion protein CD31
     > 95% positive by immunofluorescence
    Cytoplasmic VWF / Factor VIII
     > 95% positive by immunofluorescence
    Cytoplasmic uptake of Di-I-Ac-LDL
    > 95% positive by immunofluorescence

    Characterization in Peer-Reviewed Literature

    Immunofluorescence staining showing expression of endothelial (EnC)-specific markers by human glomerular EnC (GEnC) cultured on coverslips. Magnification, x200.

    Chart showing the effect of TNF-α on e-selectin expression by GEnC in a cell-based fluorescence immunoassay. GEnC were incubated with TNF-α at various concentrations or control medium for 6 h before fixing and labeling for e-selectin. E-selectin expression is proportional to fluorescence emission. Bars show mean ± SEM; n = 12; P < 0.001 by ANOVA. 

    Chart showing the results of separate experiments examining the effect of cAMP medium (containing 2 μM RO-20-1724 and 30 μM pCPT-cAMP) or thrombin (1 U/ml) on transendothelial electrical resistance (TEER) of GEnC monolayers after 1 h. Bars show mean ± SEM; n = 5, P = 0.006 for effect of cAMP; n = 8, P < 0.0005 for effect of thrombin, P values by t test. cAMP medium increased TEER by 34.4 Ω/cm2 compared with control, whereas thrombin decreased TEER by 14.8 Ω/cm2.

    Graphs showing the effect of “cAMP medium” (containing 2 μM RO-20-1724 and 300 μM pCPT-cAMP; A) or thrombin (1 U/ml; B) on passage of FITC-BSA through GEnC monolayers over time. Points show mean ± SEM, n = 10, P = 0.003 for effect of cAMP; n = 10, P < 0.0005 for effect of thrombin by repeated measures [rm] ANOVA.

    From Satchell et al (2004) J Am Soc Nephrology 15(3):566-574.


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