These antibody-free human primary cells were initiated by decapsulated glomeruli isolated from normal human kidney cortical tissue without the use of positive selection of antibodies.
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Increase Biological Relevance with Human Primary Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Isolated from normal, healthy donor tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.
A Selection of Citations for ACBRI 128 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells
- "Dipeptidyl peptidase-4 inhibitor linagliptin reduces urinary albumin excretion through the protection of glomerular endothelial function" Kondo et al. Kawasaki Medical Journal, 2021.
- "Differential procoagulatory response of microvascular, arterial and venous endothelial cells upon inflammation in vitro" Brandtner et al. Thrombosis Research, 2021.
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"Proteoglycans contribute to the functional integrity of the glomerular endothelial cell surface layer and are regulated in diabetic kidney disease" Khramova et al. Scientific Reports, 2021.
- "Lowering the increased intracellular pH of human‐induced pluripotent stem cell‐derived endothelial cells induces formation of mature Weibel‐Palade bodies" Tiemeier et al. Stem Cell Reports, 2020.
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"Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts" Cohen et al. Scientific Reports, 2020.
- "Closing the Mitochondrial Permeability Transition Pore in hiPSC-Derived Endothelial Cells Induces Glycocalyx Formation and Functional Maturation" Tiemeier et al. Stem Cell Reports, 2019.
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"Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip" Musah et al. Nature Protocols, 2018.
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"Brain microvascular endothelial cells exhibit lower activation of the alternative complement pathway than glomerular microvascular endothelial cells" Sartain et al. J Biological Chemistry, 2018.
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"Tacrolimus Prevents von Willebrand Factor Secretion by Allostimulated Human Glomerular Endothelium" Beland et al. Am J Transplantation, 2018.
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"Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip" Musah et al. Nature and Biological Engineering, 2017.
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"The formation of quiescent glomerular endothelial cell monolayer in vitro is strongly dependent on the choice of extracellular matrix coating" Pajecka et al. Experimental Cell Research, 2017.
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"The human kidney capsule contains a functionally distinct mesenchymal stromal cell population" Leuning et al. PLoS One, 2017.
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"Nitrite Activates 5′AMP-Activated Protein Kinase-Endothelial Nitric Oxide Synthase Pathway in Human Glomerular Endothelial Cells" Miyamoto et al. Biol. & Pharma Bulletin, 2017.
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"Circulating Fibroblast Growth Factor-2, HIV-Tat, and Vascular Endothelial Cell Growth Factor-A in HIV-Infected Children with Renal Disease Activate Rho-A and Src in Cultured Renal Endothelial Cells" Das et al. PLoS One, 2016.
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"TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human GLomerular Endothelial Cells" Sartain et al. J Immunology, 2016.
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"TNF causes changes in glomerular endothelial permeability and morphology through a Rho and myosin light chain kinase-dependent mechanism" Xu et al. Physiological Reports, 2015.
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"Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings" Turner and Moake, PLoS One, 2015.
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"Adenosine Kinase Inhibition Protects The Kidney Against Streptozotocin-Induced Diabetes Through Anti-inflammatory and Anti-oxidant Mechanisms" Pye et al. Pharmacological Research, 2014.
Standard Tests
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
| TESTS | RESULTS |
| Bacterial Sterility (culture method) by independent lab | Pass |
| Fungal Sterility (culture method) by independent lab | Pass |
| Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
| TESTS | RESULTS |
| Inducible expression of CD 62E (E-Selectin) | > 90% positive by immunofluorescence |
| Cell adhesion protein CD31 | > 95% positive by immunofluorescence |
| Cytoplasmic VWF / Factor VIII | > 95% positive by immunofluorescence |
| Cytoplasmic uptake of Di-I-Ac-LDL | > 95% positive by immunofluorescence |
Immunofluorescence imagery and characterization in peer reviewed literature
ACBRI 128 at Passage 6, demonstrating uptake of fluorescently labeled DiI-Ac-LDL as functional test for endothelial cell identity.
Immunofluorescence staining showing expression of endothelial (EnC)-specific markers by human glomerular EnC (GEnC) cultured on coverslips. Magnification, x200.
Chart showing the effect of TNF-α on e-selectin expression by GEnC in a cell-based fluorescence immunoassay. GEnC were incubated with TNF-α at various concentrations or control medium for 6 h before fixing and labeling for e-selectin. E-selectin expression is proportional to fluorescence emission. Bars show mean ± SEM; n = 12; P < 0.001 by ANOVA.
Chart showing the results of separate experiments examining the effect of cAMP medium (containing 2 μM RO-20-1724 and 30 μM pCPT-cAMP) or thrombin (1 U/ml) on transendothelial electrical resistance (TEER) of GEnC monolayers after 1 h. Bars show mean ± SEM; n = 5, P = 0.006 for effect of cAMP; n = 8, P < 0.0005 for effect of thrombin, P values by t test. cAMP medium increased TEER by 34.4 Ω/cm2 compared with control, whereas thrombin decreased TEER by 14.8 Ω/cm2.
Graphs showing the effect of “cAMP medium” (containing 2 μM RO-20-1724 and 300 μM pCPT-cAMP; A) or thrombin (1 U/ml; B) on passage of FITC-BSA through GEnC monolayers over time. Points show mean ± SEM, n = 10, P = 0.003 for effect of cAMP; n = 10, P < 0.0005 for effect of thrombin by repeated measures [rm] ANOVA.
From Satchell et al (2004) J Am Soc Nephrology 15(3):566-574.
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