These antibody-free human primary cells were isolated from dermis of neonatal donor without the use of positive selection of antibodies.
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- PRODUCT INFO
- CITATIONS
- TESTS
- DOCUMENTATION
Increase Biological Relevance with Human Primary Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Isolated from normal, healthy donor tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.
A Selection of Citations for CSC 2M1 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.
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"Inhibition of CaMKII in mitochondria preserves endothelial barrier function after irradiation" Roy et al. Free Radical Biology and Medicine, 2019
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"Nitroglycerin Tolerance in Caveolin-1 Deficient Mice" Mao et al. PLoS One, 2014.
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"Optical Recording Reveals Novel Properties of GSK1016790A-Induced Vanilloid Transient Receptor Potential Channel TRPV4 Activity in Primary Human Endothelial Cells" Sullivan et al. Molecular Pharmacology, 2012.
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"CCL2 Disrupts the Adherens Junction: Implications for Neuroinflammation" Roberts et al. Laboratory Investigation, 2012.
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"Anthrax Edema Toxin Inhibits Endothelial Cell Chemotaxis via Epac and Rap1" Hong et al. Journal of Biological Chemistry, 2007.
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"Endometrial Angiopoietin Expression and Modulation by Thrombin and Steroid Hormones" Krikun et al. The American Journal Of Pathology, 2004.
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"Abnormal Uterine Bleeding during Progestin-Only Contraception May Result from Free Radical-Induced Alterations in Angiopoietin Expression" Krikun et al. The American Journal of Pathology, 2002.
Standard Tests
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
| TESTS | RESULTS |
| Bacterial Sterility (culture method) by independent lab | Pass |
| Fungal Sterility (culture method) by independent lab | Pass |
| Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
| TESTS | RESULTS |
| Cytoplasmic VWF / Factor VIII | > 95% positive by immunofluorescence |
| Cytoplasmic uptake of Di-I-Ac-LDL | > 95% positive by immunofluorescence |
| Inducible expression of CD36 | > 95% positive by immunofluorescence |
COMPANION PRODUCTS:
OPTIMIZE YOUR RESEARCH
WITH HUMAN PRIMARY CELLS
Avoid the obstacles from immortalized cell lines or animal models and gain more pertinent insights into human cell biology.
Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.
Please read our Terms and Conditions