Validated Human Brain Cells for BBB, Drug Transport & Neurovascular Research

Cell Systems’ primary human brain microvascular endothelial cells (ACBRI 376) and brain pericytes (ACBRI 498) are optimized for BBB modeling, drug transport, and neurovascular research.
 
These high-viability, early passage cells are isolated without antibody selection and validated across multiple peer-reviewed studies for expression of key markers including CD31, VEGF, IL-6, and miR-296.
 
With proven performance in co-culture, angiogenesis, inflammation, and gene expression assays, they’re ideal for 2D/3D culture, organ-on-chip, and brain cell platforms.
 

Key Features:

  • Early passage cells, Passage 3 (<12 population doublings)
  • Antibody-free isolation
  • High viability (~85%), 1M cells per vial
  • Supports 2D/3D culture, organ-on-chip, and co-culture
  • Shipped as frozen vials or proliferating flasks
  • Consistency across long-term studies with deep inventory from a single donor lot.
 

Cell Profile for ACBRI 376: Surface Markers, Genes & Soluble Molecules (Show More>)

Cell Marks, Genes & Molecules Tested Methodologies Most Cited Studies

CD31, CD51, CD54, CD62E, CD105, CD106, Annexin V
  • Apoptosis Assay,
  • Cell Markers,
  • Endothelial Microparticles Preparation and Assay

Jimenez, JJ (2003). Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis 

Ang, FGFa, G-CSF, HGF, IFNg, IL-1a/b, IL-6, IL-8, IL-12, IP-10, Leptin, PIGF, TGFa, TIMP-1/2, TNFa, VEGF
  • HBMVEC in vitro angiogenesis assay,
  • Gluc mRNA translation assay,
  • PKH67 labelled microvesicle

Skog, J (2008). In vitro angiogenesis assay, Gluc mRNA translation, PKH67 vesicle tracking

CD31, miRNA (miR-181-a, miR-101, miR-30b, miR-27a, miR-21, miR-22, miR-23b, miR-31, miR-103, miR-126, miR-29a, miR-125b, miR-296)
  • RNA Isolation
  • miRNA Inhibition and Overexpression
  • In Vitro Migration Scratch Assay Quantitation of HGS, PDGFRb, and VEGFR2 Expression in Cells Treated with siRNAs
  • Vivo Imaging of Angiogenesis 

Wurdinger, T (2008). miR-296 Regulates Growth Factor Receptor Overexperssion in Angiogenic Endothelial Cells 

For details on our primary human cell profiles, contact us at customerservice@cell-systems.com

Cell Profile for ACBRI 498: Surface Markers, Genes & Soluble Molecules (Show More>)

Cell Marks, Genes & Molecules Tested Methodologies Most Cited Studies

RAGE variant proteins
  • Isolation and sequence determination of polysomal RAGE RNA splice variants (full-length and N-truncated)
  • Detection of the RAGE splice variant proteins in primary cultured human microvascular cells

Yonekura, H (2003)

Pericytes’ effects on endothelial production of G-CSF, IL-6, IL-8 and IL-17 in chips and transwell cultures
  • Blood-brain barrier
  • Microfluidic chips, fabrication and pre-treatment
  • Cell culture in three-dimensional gels
  • Permeability assay
  • Transwell cell culture Inflammatory stimulation and analysis of cytokine release

Herland, A (2016). Distinct contributions of astrocytes and pericytes to neuroinflammation Identified in a 3D human blood-brain barrier on a chip

PDGFR-β, β-Actin, Akt, NGF, BDNF, NT-3
  • PCR and Real Time PCR
  • Immunoblot Analyses
  • Akt Phosphorylation

Arimura, K (2012). PDGF receptor β signaling in pericytes following ischemic brain injury

Fibronectin +, Vimentin, CD68, NG-2

 

  • Real Time qPCR

  • Cytomegalovirus Infraction

Wilkerson, I (2015). Retinal pericytes and cytomegalovirus infectivity: implications for HCMV-induced retinopathy and congenital ocular disease

For details on our primary human cell profiles, contact us at customerservice@cell-systems.com

Order HRMEC Cells and Pericytes Online

Order brain endothelial cells online or submit a purchase order to order@cell-systems.com. Pair them with media and reagents specifically tested for consistent performance with Cell Systems' brain endothelial and pericyte cells.

Cell Systems Primary Human Aortic Endothelial Cells (ACBRI 375)

Primary Human Brain Microvascular Endothelial Cells (ACBRI 376)

Isolated from normal human brain cortex

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Cell Systems Primary Human Aortic Endothelial Cells (ACBRI 375)

Primary Human Brain Pericytes Cells (ACBRI 498)

Isolated from normal human cerebral cortex  

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Optimized Media & Reagents for Brain Cell Cultures

Cell Systems Primary Human Aortic Endothelial Cells (ACBRI 375)

Complete Classic Medium With Serum and CultureBoost™ (4Z0-500)

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Cell Systems Primary Human Aortic Endothelial Cells (ACBRI 375)

Attachment Factor™ (4Z0-201/4Z0-210)

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Cell Systems Primary Human Aortic Endothelial Cells (ACBRI 375)

The System™ (CSS-A101) for Cell Cultures

Online Special: Save $200 with the Purchase of Primary Cells. Automatic discount at checkout.

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Validated across dozens of peer-reviewed studies—from microfluidic BBB models to stroke and neuroinflammation research.
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.

Validated across peer-reviewed studies—from BBB models to inflammation, vascular function, and neural applications.
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.