Validated Human Brain Cells for BBB, Drug Transport & Neurovascular Research

Cell Systems’ primary human brain microvascular endothelial cells (ACBRI 376) and brain pericytes (ACBRI 498) are optimized for BBB modeling, drug transport, and neurovascular research.
 
These high-viability, early passage cells are isolated without antibody selection and validated across multiple peer-reviewed studies for expression of key markers including CD31, VEGF, IL-6, and miR-296.
 
With proven performance in co-culture, angiogenesis, inflammation, and gene expression assays, they’re ideal for 2D/3D culture, organ-on-chip, and brain cell platforms.
 

Key Features:

  • Early passage cells, Passage 3 (<12 population doublings)
  • Antibody-free isolation
  • High viability (~85%), 1M cells per vial
  • Supports 2D/3D culture, organ-on-chip, and co-culture
  • Shipped as frozen vials or proliferating flasks
  • Consistency across long-term studies with deep inventory from a single donor lot.
 

Expression Profile for ACBRI 376: Surface Markers, Genes & Soluble Molecules >

Cell Marks, Genes & Molecules Tested Methodologies Most Cited Studies

CD31, CD51, CD54, CD62E, CD105, CD106, Annexin V

  • Apoptosis Assay,
  • Cell Markers,
  • Endothelial Microparticles Preparation and Assay

Ang, FGFa, G-CSF, HGF, IFNg, IL-1a/b, IL-6, IL-8, IL-12, IP-10, Leptin, PIGF, TGFa, TIMP-1/2, TNFa, VEGF

  • HBMVEC in vitro angiogenesis assay,
  • Gluc mRNA translation assay,
  • PKH67 labelled microvesicle

CD31, miRNA (miR-181-a, miR-101, miR-30b, miR-27a, miR-21, miR-22, miR-23b, miR-31, miR-103, miR-126, miR-29a, miR-125b, miR-296)

  • RNA Isolation
  • miRNA Inhibition and Overexpression
  • In Vitro Migration Scratch Assay Quantitation of HGS, PDGFRb, and VEGFR2 Expression in Cells Treated with siRNAs
  • Vivo Imaging of Angiogenesis 

Expression Profile for ACBRI 498: Surface Markers, Genes & Soluble Molecules >

Cell Marks, Genes & Molecules Tested Methodologies Most Cited Studies

Order HRMEC Cells and Pericytes Online

Order brain endothelial cells online or submit a purchase order to order@cell-systems.com. Pair them with media and reagents specifically tested for consistent performance with Cell Systems' brain endothelial and pericyte cells.

Isolated from normal human brain cortex

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Isolated from normal human cerebral cortex  

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Optimized Media & Reagents for Brain Cell Cultures

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Online Special: Save $200 with the Purchase of Primary Cells. Automatic discount at checkout.

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Validated across dozens of peer-reviewed studies—from microfluidic BBB models to stroke and neuroinflammation research.
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.

Validated across peer-reviewed studies—from BBB models to inflammation, vascular function, and neural applications.
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.