The Serum-Free Recombinant Growth Factor System is a complete cell growth and passage system including 2 x 500mL SF-4Z0-500-R Cell Systems Serum-Free Medium, 1 x 100 mL Attachment Factor™, 2 x 8mL RocketFuel-R™, 1 set 100mL Passage Reagent Group™ and 1 x 50mL Cell Freezing Medium™. Contains Recombinant Growth Factors only.

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Primary Human Glomerular Mesangial Cells (ACBRI 127)

THE SYSTEM™ is an all-in-one cell growth and passage system designed to provide your lab with convenience and consistency.


Medium Component

Serum-Free Medium - does not contain bovine derived components. Human serum albumin and other components of human origin are specified by the formulation. This medium becomes complete once activated with the included RocketFuel-R™ supplement containing Recombinant Growth Factors only. This medium is recommended for use with a stable proliferating culture when Serum-Free conditions are required for experimentation. For thawing and plating of cryopreserved cells, we recommend Media With Serum.


Growth Supplement Component

RocketFuel-R™ is the broad-spectrum mitogen growth supplement containing only human recombinant growth factors, designed to provide defined growth supplements in the serum-free media environment.


PRG Component

Passage Reagent Group™ (PRG) is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1(EDTA-dPBS Solution), PRG-2 (Trypsin/EDTA-dPBS Solution) and PRG-3(Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1 prepares for PRG-2, which contains highly purified trypsin. PRG-3 inactivates the protease in PRG-2 and stabilizes the cell membranes.  


Cell Freezing Medium™ Component

Cell Systems Cell Freezing Medium™ is a specialized media, which when used in conjunction with Passage Reagent Group™ provides a beneficial environment for the freeze/thaw cycle of cell cultures, assisting in the minimization of cellular damage during the process.


Attachment Factor™ Component

Attachment Factor™ is an extracellular matrix (ECM) product that promotes cell attachment to tissue culture surfaces and encourages correct polarity and cytoskeletal organization. Use of Attachment Factor™ is critically important when cultures are to be initiated, grown, passaged, or used within the Cell Systems Media family. See the Attachment Factor™ protocol.

Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.


Avoid the obstacles from immortalized cell lines or animal models and gain more pertinent insights into human cell biology.