The Serum-Free Recombinant Growth Factor System is a complete cell growth and passage system including 2 x 500mL SF-4Z0-500-R Cell Systems Serum-Free Medium, 1 x 100 mL Attachment Factor™, 2 x 8mL RocketFuel-R™, 1 set 100mL Passage Reagent Group™ and 1 x 50mL Cell Freezing Medium™. Contains Recombinant Growth Factors only.
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THE SYSTEM™ is an all-in-one cell growth and passage system designed to provide your lab with convenience and consistency.
Serum-Free Medium - does not contain bovine derived components. Human serum albumin and other components of human origin are specified by the formulation. This medium becomes complete once activated with the included RocketFuel-R™ supplement containing Recombinant Growth Factors only. This medium is recommended for use with a stable proliferating culture when Serum-Free conditions are required for experimentation. For thawing and plating of cryopreserved cells, we recommend Media With Serum.
Growth Supplement Component
RocketFuel-R™ is the broad-spectrum mitogen growth supplement containing only human recombinant growth factors, designed to provide defined growth supplements in the serum-free media environment.
Passage Reagent Group™ (PRG) is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1(EDTA-dPBS Solution), PRG-2 (Trypsin/EDTA-dPBS Solution) and PRG-3(Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1 prepares for PRG-2, which contains highly purified trypsin. PRG-3 inactivates the protease in PRG-2 and stabilizes the cell membranes.
Cell Freezing Medium™ Component
Cell Systems Cell Freezing Medium™ is a specialized media, which when used in conjunction with Passage Reagent Group™ provides a beneficial environment for the freeze/thaw cycle of cell cultures, assisting in the minimization of cellular damage during the process.
Attachment Factor™ Component
Attachment Factor™ is an extracellular matrix (ECM) product that promotes cell attachment to tissue culture surfaces and encourages correct polarity and cytoskeletal organization. Use of Attachment Factor™ is critically important when cultures are to be initiated, grown, passaged, or used within the Cell Systems Media family. See the Attachment Factor™ protocol.
Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.