Cell Systems News — Biological Relevance

3D models are often applauded for being more predictive than 2D cultures, but not all scientists know how to maximize their 3D models’ biological relevance to reach their full potential. Using primary human cells (derived directly from human tissues) can greatly enhance the biological relevance of your models and steer your experiments in the right direction from the start. In a study by Kim et al., researchers use primary liver sinusoidal endothelial cells (Cat# ACBRI 566) to construct a 3D microfluidic human liver-chip to better understand how breast cancer-derived extracellular vehicles (EVs) can influence metastasis in the liver.

Using primary human cells in your in vitro models can greatly enhance the biological relevance of your research, resulting in real human insights and saving time and money down the road. Cell Systems Primary Human Glomerular Endothelial Cells (Cat#. ACBRI 128) were used in a human in vitro model by the University Health Center of Quebec to better understand chronic antibody-mediated rejection (ABMR) in kidney transplant recipients. Across multiple experiments in the study, the results gathered from the primary glomerular cell model show similar patterns to what was observed in kidney transplant recipients.

3D modeling of the blood-brain barrier in a microfluidic chip using Cell Systems human primary cells has shed light onto how different types of brain cells can independently affect the brain’s response to neuroinflammation. As a more physiologically relevant model, this BBB-chip can be a new promising tool for understanding the brain.