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Primary Human Glomerular Mesangial Cells (ACBRI 127)⎢Cell Systems

Regular price $735.00

Primary Human Glomerular Mesangial Cells (ACBRI 127) were initiated by elutriation of dispase dissociated decapsulated glomeruli isolated from normal human cortical kidney tissue.



Cell Initiation

These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask.  In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).

Companion Products

Each vial of cells is shipped with Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.

These cells are qualified for use with:

Complete Classic Medium Kit with Serum and CultureBoost™
Complete Classic Medium Kit with Serum and CultureBoost™

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The System | All-in-One Kit
The System™

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Passage Reagent Group
Passage Reagent Group™

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Standard Tests

TEST RESULTS
HIV Serologic Test (donor level HIV AB EIA) Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab) Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) Negative

Miscellaneous Tests

TEST RESULTS
Inducible expression of CD 62E (E-Selectin)  < 2% positive by immunofluorescence
Cytoplasmic Vimentin Intermediate Filaments  > 98% positive by immunofluorescence
Cytoplasmic Alpha-Actinin Microfilaments  > 98% positive by immunofluorescence
Cytoplasmic Fibronectin 
> 98% positive by immunofluorescence

Citations


    ACBRI cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. ACBRI cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of ACBRI materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization. 

    The Applied Cell Biology Research Institute (ACBRI) was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching.  It provided cells and cell culture products to other research entities - public and private. Cell Systems is the custodian of all ACBRI materials.

    Please read our Terms and Conditions