These antibody-free human primary cells were isolated by elutriation of dispase dissociated decapsulated glomeruli isolated from normal human cortical kidney tissue without the use of positive selection of antibodies.
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- PRODUCT INFO
- CITATIONS
- TESTS
- DOCUMENTATION
Increase Biological Relevance with Human Primary Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Isolated from normal, healthy donor tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.
A Selection of Citations for ACBRI 127 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.
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"Ameliorative effect of phosphodiesterase 4 and 5 inhibitors in deoxycorticosterone acetate-salt hypertensive uni-nephrectomized KKAy mice" Nio and Ookawara et al. FASEB J, 2020.
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"Study of the target effect of mannose modified liposomes on diabetic rat kidney based on GLUT" Yang et al. Journal of Drug Delivery Science and Technology, 2020.
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"Cell Surface Protein Disulfide Isomerase Regulates Natriuretic Peptide Generation of Cyclic Guanosine Monophosphate" Pan et al. PLoS One, 2014.
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"The combined role of galactose-deficient IgA1and streptococcal IgA-binding M protein in inducing IL-6 and C3 secretion from human mesangial cells: implications for IgA nephropathy" Schmitt et al. J Immunology, 2014.
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"Promoter methylation confers kidney-specific expression of the Klotho gene" Azuma et al. FASEB J, 2012.
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"Nicotine worsens the severity of nephropathy in diabetic mice: implications for the progression of kidney disease in smokers" Hua et al. Renal Physiology, 2010.
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"Nicotine: the link between cigarette smoking and the progression of renal injury?" Jaimes et al. Am J Physiol Heart Circ Physiol, 2007.
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"Blocking of α5 integrin stimulates production of TGF-β and PAI-1 by human mesangial cells" Matsumoto et al. Elsevier, 2003
Standard Tests
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
| TESTS | RESULTS |
| Bacterial Sterility (culture method) by independent lab | Pass |
| Fungal Sterility (culture method) by independent lab | Pass |
| Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
| TESTS | RESULTS |
| Inducible expression of CD 62E (E-Selectin) | < 2% by immunofluorescence |
| Cytoplasmic Vimentin Intermediate Filaments | > 98% positive by immunofluorescence |
| Cytoplasmic Alpha-Actinin Microfilaments | > 98% positive by immunofluorescence |
| Cytoplasmic Fibronectin | > 98% positive by immunofluorescence |
COMPANION PRODUCTS:
OPTIMIZE YOUR RESEARCH
WITH HUMAN PRIMARY CELLS
Avoid the obstacles from immortalized cell lines or animal models and gain more pertinent insights into human cell biology.
Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.
Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.
Please read our Terms and Conditions