The System Without Serum™ and With CultureBoost™ (CSS-A104)
The System™ Without Serum. Cell growth and passage system including 2 x 4Z3-500 500mL Mediums formulated without serum, 1 x 100mL Attachment Factor™, 2 x 5mL CultureBoost™, 1 set 100mL Passage Reagent Group™ and 1 x 50mL Cell Freezing Medium.
Medium - formulated without serum. This medium requires the addition of serum by the end-user according to experimentation requirements. This medium becomes complete once supplemented with serum (not included) and activated with CultureBoost™ supplement (included). 4Z3-500 is certified and intended for experimental application.
Growth Supplement Component
CultureBoost™ is the broad-spectrum supplement used to activate CSC Complete Medium. CultureBoost™ contains Cell Systems bovine Growth Factor and porcine heparin.
Passage Reagent Group™ (PRG) is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1(EDTA-dPBS Solution), PRG-2 (Trypsin/EDTA-dPBS Solution) and PRG-3 (Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1 prepares for PRG-2, which contains highly purified trypsin. PRG-3 inactivates the protease in PRG-2 and stabilizes the cell membranes.
Cell Freezing Medium™ Component
Cell Systems Cell Freezing Medium™ is a specialized media, which when used in conjunction with Passage Reagent Group™ provides a beneficial environment for the freeze/thaw cycle of cell cultures, assisting in the minimization of cellular damage during the process.
Attachment Factor™ Component
Attachment Factor™ is an extracellular matrix (ECM) product that promotes cell attachment to tissue culture surfaces and encourages correct polarity and cytoskeletal organization. Use of Attachment Factor™ is critically important when cultures are to be initiated, grown, passaged, or used within the Cell Systems Medium family. See the Attachment Factor™ protocol.
Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.