The System™ Without Serum. Cell growth and passage system including 2 x 4Z3-500 500mL Mediums formulated without serum, 1 x 100mL Attachment Factor™, 2 x 5mL CultureBoost™, 1 set 100mL Passage Reagent Group™ and 1 x 50mL Cell Freezing Medium.

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Primary Human Glomerular Mesangial Cells (ACBRI 127)

THE SYSTEM™ is an all-in-one cell growth and passage system designed to provide your lab with convenience and consistency.


Medium Component

Medium - formulated without serum. This medium requires the addition of serum by the end-user according to experimentation requirements. This medium becomes complete once supplemented with serum (not included) and activated with CultureBoost™ supplement (included). 4Z3-500 is certified and intended for experimental application.


Growth Supplement Component

CultureBoost™ is the broad-spectrum supplement used to activate CSC Complete Medium. CultureBoost™ contains Cell Systems bovine Growth Factor and porcine heparin.


PRG Component

Passage Reagent Group™ (PRG) is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1(EDTA-dPBS Solution), PRG-2 (Trypsin/EDTA-dPBS Solution) and PRG-3(Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1 prepares for PRG-2, which contains highly purified trypsin. PRG-3 inactivates the protease in PRG-2 and stabilizes the cell membranes.  


Cell Freezing Medium™ Component

Cell Systems Cell Freezing Medium™ is a specialized media, which when used in conjunction with Passage Reagent Group™ provides a beneficial environment for the freeze/thaw cycle of cell cultures, assisting in the minimization of cellular damage during the process.


Attachment Factor™ Component

Attachment Factor™ is an extracellular matrix (ECM) product that promotes cell attachment to tissue culture surfaces and encourages correct polarity and cytoskeletal organization. Use of Attachment Factor™ is critically important when cultures are to be initiated, grown, passaged, or used within the Cell Systems Media family. See the Attachment Factor™ protocol.


Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.


Avoid the obstacles from immortalized cell lines or animal models and gain more pertinent insights into human cell biology.