Cell Systems⎢The System Without Serum™ and With CultureBoost™ (CSS-A104)
The System™ Without Serum. Cell growth and passage system including 2-ea 4Z3-500 500mL Mediums formulated without serum, 1-ea 100mL Attachment Factor™, 2-ea 10mL CultureBoost™, 1 set 100mL Passage Reagent Group™ and 1-ea 50mL Cell Freezing Medium.
Medium - formulated without serum. This medium is intended for use with Serum, add the type and percentage of serum appropriate to experimentation requirements. This medium becomes complete once activated with the included CultureBoost™ supplement. 4Z3-500 is Certified and intended for experimental application.
Growth Supplement Component
CultureBoost™ is the broad-spectrum supplement used to activate CSC Complete Medium. CultureBoost™ contains Cell Systems bovine Growth Factor and Porcine heparin.
Passage Reagent Group™ (PRG) is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1(EDTA -dPBS Solution), PRG-2 (Trypsin/EDTA -dPBS Solution) and PRG-3 (Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1 prepares for PRG-2, which contains highly purified trypsin. PRG-3 inactivates the protease in PRG-2 and stabilizes the cell membranes.
Cell Freezing Medium™ Component
Cell Systems Cell Freezing Medium™ is a specialized media, when used in conjunction with Passage Reagent Group™, provides a beneficial environment for the freeze/thaw cycle of cell cultures, assisting in the minimization of cellular damage during the process.
Attachment Factor™ Component
Attachment Factor™ is an extracellular matrix (ECM) product that promotes cell attachment to tissue culture surfaces and encourages correct polarity and cytoskeletal organization. Attachment Factor™ also contributes to correct in vitro growth factor and biological response modifier presentation. Use of Attachment Factor™ is critically important when cultures are to be initiated, grown, passaged, or used within the Cell Systems Medium family.
Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.